Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9) (CAT#: PABX-101)

Figure 1 SDS-PAGEs of fed-batch culture supernatants under reducing (left panel) and non reducing (right panel) conditions from CHO-K1/PG9 cell line at different days (D4-D12, PG9 St: antibody standard).

Zboray, K., Sommeregger, W., Bogner, E., Gili, A., Sterovsky, T., Fauland, K., ... & Kunert, R. (2015). Heterologous protein production using euchromatin-containing expression vectors in mammalian cells. Nucleic acids research, 43(16), e102-e102.

Figure 2 BAC-based PG9 antibody fed-batch production in shake flasks and bioreactors.

(A) Product accumulation (mg/l) and (B) viable cell densities (viable cells/ml) in shake flask and bioreactor fed-batch cultures. (C) Specific (pcd) and volumetric (g/ml) productivity of CHO-K1/PG9 cell line in routine cultivation without selection pressure over 20 passages (73 days). (D) Binding curve of PG9 and negative control 2F5 to gp120 (ZM109), PG9 EC50 was calculated to be 64 ng/ml.

Zboray, K., Sommeregger, W., Bogner, E., Gili, A., Sterovsky, T., Fauland, K., ... & Kunert, R. (2015). Heterologous protein production using euchromatin-containing expression vectors in mammalian cells. Nucleic acids research, 43(16), e102-e102.

Figure 3 Purification and characterization of the PG9 Fab:BG505 SOSIP.664 complex.

(A) Size exclusion chromatography (SEC) profile of the PG9 Fab:BG505 SOSIP.664 gp140 complex in the presence of excess PG9 Fab. (B) The observed shifts in elution volume and molar mass of the protein (MMglycoprotein) for the complex (red), compared with the unliganded trimer (blue), indicate that one PG9 Fab binds per trimer. The horizontal line under each peak corresponds to the MMglycoprotein of the eluting sample as determined by SEC-UV/MALS/RI. These results are summarized in Table S1. (C) Nonreducing SDS/PAGE gel analysis of the SEC fractions. PG9 Fab and BG505 SOSIP.664 were stained similarly in control lanes when the same amount of protein was used. ImageJ (59) was used to determine that, in the eluting samples, one PG9 Fab associates with three gp140 moieties (1:3 ratio).

Julien, J. P., Lee, J. H., Cupo, A., Murin, C. D., Derking, R., Hoffenberg, S., ... & Sanders, R. W. (2013). Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9. Proceedings of the National Academy of Sciences, 110(11), 4351-4356.