Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT145) (CAT#: PABX-098)

Anti-HIV-1 antibody is a Human antibody of IgG class that binds to an HIV-1.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Binding kinetics for BG505.SOSIP and 15-sc gp140 with VRC26.09 and PGT145.

Figure 1 Binding kinetics for BG505.SOSIP and 15-sc gp140 with VRC26.09 and PGT145.

Georgiev, I. S., Joyce, M. G., Yang, Y., Sastry, M., Zhang, B., Baxa, U., ... & Schön, A. (2015). Single-chain soluble BG505. SOSIP gp140 trimers as structural and antigenic mimics of mature closed HIV-1 Env. Journal of virology, 89(10), 5318-5329.

Figure 2 Binding of SOSIP and SOSIP-sfGFP proteins in HEK293T cell supernatant to bNAbs 2G12 and PGT145 as assessed by ELISA.

Figure 2 Binding of SOSIP and SOSIP-sfGFP proteins in HEK293T cell supernatant to bNAbs 2G12 and PGT145 as assessed by ELISA.

SOSIP and SOSIP-sfGFP proteins were captured onto the solid phase.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.

Figure 3  Coomassie-stained reducing and non-reducing SDS-PAGE gels showing SOSIP and SOSIP-sfGFP, both purified with a PGT145-affinity column.

Figure 3 Coomassie-stained reducing and non-reducing SDS-PAGE gels showing SOSIP and SOSIP-sfGFP, both purified with a PGT145-affinity column.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.

Figure 4 Antigenicity characterization by ECLIA. An extended panel of both broadly and poorly neutralizing antibodies was assessed using ECLIA for cleaved (black open circles) and 15-sc gp140 (red filled circles) BG505.SOSIP constructs.

Figure 4 Antigenicity characterization by ECLIA. An extended panel of both broadly and poorly neutralizing antibodies was assessed using ECLIA for cleaved (black open circles) and 15-sc gp140 (red filled circles) BG505.SOSIP constructs.

Sliepen, K., Van Montfort, T., Ozorowski, G., Pritchard, L., Crispin, M., Ward, A., & Sanders, R. (2015). Engineering and characterization of a fluorescent native-like HIV-1 envelope glycoprotein trimer. Biomolecules, 5(4), 2919-2934.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Tested positive against HIV-1
  • Species Reactivity
  • HIV-1
  • Clone
  • PGT145
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (Assay-dependent)

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Assay-dependent

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone PGT145"

See other products for "HIV-1 gp120"

Single-domain Antibody

Recombinant Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABX-098. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare