Recombinant Human Anti-HIV-1 Antibody scFv Fragment (447-52D) (CAT#: PABX-088-S (P))

Figure 1 Binding of the mutated 447-52D IgG to the indicated gp142 trimers as measured by ELISA.

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-54D (determined in panel A).

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2221 (blue).

The psV consist of the SF162 strain with its V3 loop replaced by the indicated V3 loop sequence. Consensus B-R18Q is a psV consisting of the consensus subtype B V3 loop sequence with position 18 in the V5 loop mutated from a subtype B consensus arginine t

Almond, D., Krachmarov, C., Swetnam, J., Zolla-Pazner, S., & Cardozo, T. (2012). Resistance of subtype C HIV-1 strains to anti-V3 loop antibodies. Advances in virology, 2014.