Mouse Anti-HIV-1 P24 Recombinant Antibody (clone 13B5) (CAT#: PABZ-069)

Recombinant Mouse Antibody (13B5) is capable of binding to HIV-1 P24, expressed in mammalian cells. This antibody recognizes a nearly continuous epitope comprising a helix-turn-helix motif in the C-terminal domain of p24.


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Figure 1 Epitope accessibility on PLA-p24 nanoparticles.

Figure 1 Epitope accessibility on PLA-p24 nanoparticles.

Competitive ELISA curves representing OD as a function of free (black diamonds) or PLA-adsorbed (gray diamonds) p24 concentration were obtained for six mAbs used at an optimal concentration, as indicated on each panel. 13B5 targets the C-terminal domain of p24. A rapid decrease of OD signal indicates efficient competition and optimal accessibility of the epitope. A slow or no decrease reflects inefficient competition and poor accessibility.

Lamalle-Bernard, D., Munier, S., Compagnon, C., Charles, M. H., Kalyanaraman, V. S., Delair, T., ... & Ataman-Önal, Y. (2006). Coadsorption of HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles preserves antigenicity and immunogenicity. Journal of controlled release, 115(1), 57-67.


Specifications

  • Immunogen
  • HIV-1 P24 capsid protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Tested positive against HIV-1 P24
  • Species Reactivity
  • HIV-1
  • Clone
  • 13B5
  • Applications
  • ELISA Assay-Dependent
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:100-1:500
    Neut Assay-Dependent

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody 13B5 has been reported in applications of ELISA, WB, IHC, IF, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
    ELISA: The reported concentration of 13B5 for competitive ELISA was 3 ng/ml.

Target

  • Alternative Names
  • P24 capsid protein; P24; p24 (HIV-1)

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 13B5"

See other products for "HIV-1 p24"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1613 Rabbit Anti-HIV-1 p24 Recombinant Antibody (clone DS1613AB) WB Rabbit IgG

Single-domain Antibody

CAT Product Name Application Type
PABC-580 Recombinant Llama Anti-HIV-1 p24 Single Domain Antibody (VHH 59H10) ELISA Llama VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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