Recombinant Mouse Antibody (0. 5β) is capable of binding to HIV-1 gp120, expressed in mammalian cells. The affinity of 0. 5β for the V3 peptide P1053 (RKSIRIQRGPGRAFVTIG) was found to be comparable to its affinity for the intact glycoprotein.
Figure 1 Titration of the 0.5β Fv fragment with wild-type peptide monitored by fluorescence enhancement.
The concentration of the 0.5β Fv fragment was 0.5 μM.
Faiman, G. A., Levy, R., Anglister, J., & Horovitz, A. (1996). Contribution of Arginine Residues in the RP135 Peptide Derived from the V3 Loop of gp120 to Its Interaction with the Fv Fragment of the 0.5 β HIV-1 Neutralizing Antibody. Journal of Biological Chemistry, 271(23), 13829-13833.
Figure 2 Neutralization of the HTLV-IIB and the Mutant P313-A Viruses by V3 Loop-Specific Antisera
The neutralization titers represent the dilution of antisera which inhibited RT release from virus challenged cells by 90%. 9284 and 0.5β are monoclonal antibodies to the HTLVIIIB virus which have been shown to bind the V3 loop region with different specificity.
IVANOFF, L. A., LOONEY, D. J., McDANAL, C. H. A. R. L. E. N. E., MORRIS, J. F., WONG-STAAL, F. L. O. S. S. I. E., LANGLOIS, A. J., ... & MATTHEWS, T. J. (1991). Alteration of HIV-1 infectivity and neutralization by a single amino acid replacement in the V3 loop domain. AIDS research and human retroviruses, 7(7), 595-603.
Figure 3 Comparison of the cell fusion blocking activity of V3 loop-specific antisera on HIV-1 infected T cells.
CEM T cells chronically infected with either the HTLV-IIIB virus or the P313-A mutant virus were mixed with uninfected MOLT-4 T cells in the presence of various antisera (see legend in Table 1 for a description). After 24 h, multinucleated syncytia were enumerated. Open symbols, HTLV-IIIB virus; Closed symbols, mutant P313-A virus. (A) Comparative activity of three polyclonal antisera: anti-PB1 (open boxes, closed boxes) anti-RP135 (open triangles, closed triangles); and anti-P-A peptide (open circles, closed circles). Preimmune goat and guinea pig had no effect on syncytia number. (B) Relative activity of two monoclonal antibodies: 9284 (open circles, closed circles) and 0.5β(open boxes, closed boxes).
IVANOFF, L. A., LOONEY, D. J., McDANAL, C. H. A. R. L. E. N. E., MORRIS, J. F., WONG-STAAL, F. L. O. S. S. I. E., LANGLOIS, A. J., ... & MATTHEWS, T. J. (1991). Alteration of HIV-1 infectivity and neutralization by a single amino acid replacement in the V3 loop domain. AIDS research and human retroviruses, 7(7), 595-603.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135); scFv Fragment | ELISA, Neut | Human scFv |
PSBL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d); scFv Fragment | WB, ELISA, FuncS | Human scFv |
PFBZ-164 | Human Anti-HIV-1 gp120 Recombinant Antibody; Fab Fragment (PFBZ-164) | Neut | Human Fab |
PFBZ-165 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone GL-VRC01); Fab Fragment | Neut | Human Fab |
PSBL-532 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT122); scFv Fragment | Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135); Fab Fragment | ELISA, Neut | Human Fab |
PSBW-160 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105); scFv Fragment | FC, Neut | Human scFv |
PSBW-178 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone b12); scFv Fragment | Neut, ELISA | Human scFv |
FAMAB-0177CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone BAT123); Fab Fragment | Neut | Chimeric (mouse/human) Fab |
FAMAB-0178CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone C beta 1); Fab Fragment | WB, Neut | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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