Recombinant Human Anti-HIV-1 gp120 Antibody (2G12) (CAT#: PABC-062)

Figure 1 Human Anti-HIV-1 gp120 Antibody (2G12) in Neut.

Inhibition of the binding of 2G12 mAb to HIV-1-infected MT-4 cells in the presence of GNA, HHA and CV-N. MT-4 cells infected with HIV-1 strains IIIB (left panel), MN (middle panel) and NDK (right panel), were incubated with 2G12 mAb in the absence (red histograms) or presence of different concentrations of GNA (green histograms), HHA (orange histograms) and CV-N (light blue). The dark blue histograms show the background fluorescence. The mean fluorescence intensity (MFI) values are indicated in each histogram.

Schols, D. (2010). Microvirin, a novel (1, 2)-mannose-specific lectin isolated from Microcystis aeruginosa, has anti-HIV-1 activity comparable with that of cyanovirin-N but a much higher safety profile. proteins, 1, 3.

Figure 1 Human Anti-HIV-1 gp120 Recombinant Antibody (PABC-062) in SDS-PAGE

SDS-PAGE analysis of PABC-062 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, the different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of PABC-062 was greater than 95% as determined by SDS-PAGE.

Figure 2 Human Anti-HIV-1 gp120 Recombinant Antibody (PABC-062) in SEC-HPLC

The purity of PABC-062 was 98.9383% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-HIV-1 gp120 Recombinant Antibody (PABC-062) in WB

Western blot analysis of PABC-062 was performed by loading recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-062 and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Human Anti-HIV-1 gp120 Recombinant Antibody (PABC-062) in ELISA

ELISA analysis of PABC-062 was performed by coating with recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag). Then blocked with BSA and incubated with PABC-062. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.