Human Anti-HIV-1 gp120 Recombinant Antibody (clone E51) (CAT#: PABW-063)

Recombinant Human Antibody (E51) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.


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Figure 1 In vitro neutralization by combinations of CD4bs antibodies with CD4i antibodies.

Figure 1 In vitro neutralization by combinations of CD4bs antibodies with CD4i antibodies.

(A) HIV-1 isolates 89.6, ADA, and SG3 were preincubated for 1 h with a fixed total concentration (0.3 ug/ml for 89.6 and ADA and 0.005 g/ml for SG3) but various ratios of VRC01-E51 mixtures. TZM-bl cells were then added and incubated for 48 h. Infection was measured by the percent luciferase expression in the absence of inhibitor. (B) Experiment similar to that for panel A except that the indicated isolates were preincubated for 1 h with a fixed total concentration (0.3 ug/ml for 89.6 and ADA and 1.0 ug/ml for SA32) but various ratios of VRC01-412d mixtures. Error bars represent standard error of mean (SEM).

Gardner, M. R., Fellinger, C. H., Prasad, N. R., Zhou, A. S., Kondur, H. R., Joshi, V. R., ... & Farzan, M. (2016). CD4-induced antibodies promote association of the HIV-1 envelope glycoprotein with CD4-binding site antibodies. Journal of virology, JVI-00803.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Tested positive against HIV-1 gp120
  • Species Reactivity
  • HIV-1
  • Clone
  • E51
  • Applications
  • Neut

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 gp120 antibody has been reported in applications of Neutralization.
    ELISA: Enzyme-linked immunosorbent assay (ELISA) plates (Costar) were coated with 5 ug/ml HIV-1 gp120 and left overnight at 4°C. Plates were washed with PBS plus 0.05% Tween 20 (PBS-T) twice and blocked with 5% milk in PBS for 1 h at 37°C. Dilutions of murine E51, murine 412d, CD4-Ig, or CD4bs antibodies blocked with 5% milk in PBS were added to the plate and incubated for 1 h at 37°C.

Target

  • Alternative Names
  • ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Single-domain Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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