Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone G3-519) (CAT#: PABW-064)

Recombinant Mouse Antibody (G3-519) is capable of binding to HIV-1 gp120, expressed in mammalian cells. G3-519,recognizes an epitope from the fourth conserved region (C4) of gp120 that plays an important role in interaction with CD4. It neutralizes various HIV-1 strains in vitro.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 ELISA binding curves of select anti-gp120 Abs against full-length and loop-deleted constructs of gp140JR-FL.

Figure 1 ELISA binding curves of select anti-gp120 Abs against full-length and loop-deleted constructs of gp140JR-FL.

Full-length (diamonds), V1-deleted (squares), V2-deleted (triangles), and V3-deleted (x) gp140JR-FL were captured via the C-terminal region of gp120 with the sheep Ab D7324 and probed with pooled HIVIG, 2G12, Fab AH48, G3-4, G3-519, G3-42, G3-299, and 4KG5. The detection reagents were ABTS for 4KG5 and AP staining solution for all other Abs.

Zwick, M. B., Kelleher, R., Jensen, R., Labrijn, A. F., Wang, M., Quinnan, G. V., ... & Burton, D. R. (2003). A novel human antibody against human immunodeficiency virus type 1 gp120 is V1, V2, and V3 loop dependent and helps delimit the epitope of the broadly neutralizing antibody immunoglobulin G1 b12. Journal of virology, 77(12), 6965-6978.

Figure 2 Selective inhibition of the alternative complement activity in human whole blood by MAb 166-32 at 18 μg/mL in the extra-corporeal circulation studies before recirculation.

Figure 2 Selective inhibition of the alternative complement activity in human whole blood by MAb 166-32 at 18 μg/mL in the extra-corporeal circulation studies before recirculation.

MAb 166-32 did not inhibit the classical complement activity of the human plasma in the circuits. The negative control MAb G3-519 had no effects on either the alternative or the classical complement activity. Values are given as means ± SD from 5 paired experiments of the test and control circuits. Percentage of hemolysis was defined as indicat-ed in the text. P values were determined by means of paired t tests between MAb 166–32– and MAb G3–519–treated circuits in the alternative complement hemolytic assays.

Fung, M., Loubser, P. G., Ündar, A., Mueller, M., Sun, C., Sun, W. N., ... & Fraser Jr, C. D. (2001). Inhibition of complement, neutrophil, and platelet activation by an anti-factor D monoclonal antibody in simulated cardiopulmonary bypass circuits. The Journal of Thoracic and Cardiovascular Surgery, 122(1), 113-122.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Tested positive against HIV-1 gp120
  • Species Reactivity
  • HIV-1
  • Clone
  • G3-519
  • Applications
  • Neut

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 gp120 antibody has been reported in applications of ELISA, Inhibition.
    ELISA: Microwells were coated overnight at 4°C with 50 μl of PBS containing gp120JR-FL (2 μg/ml). Wells were washed twice with PBS containing 0.05% Tween 20 and blocked with 3% BSA for 1 h at 37°C. After a single wash, Abs were added to the wells in PBS containing 1% BSA and 0.02% Tween and allowed to incubate at 37°C for 2 h.

Target

  • Alternative Names
  • ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone G3-519"

See other products for "HIV-1 gp120"

Single-domain Antibody

Mouse Antibody

CAT Product Name Application Type
MRO-132LC Anti-HIV-1 gp120 Recombinant Antibody (BAT123) ELISA, WB, IF, Neut Mouse antibody
MRO-133LC Anti-HIV-1 gp120 Recombinant Antibody (BAT267) ELISA, WB, Neut Mouse antibody
MRO-134LC Anti-HIV-1 gp120 Recombinant Antibody (BAT085) ELISA, WB, IF, Neut Mouse antibody
MRO-150LC Anti-HIV-1 gp120 Recombinant Antibody (G3-4) ELISA, Inhib, FC, Neut, RIPA, DB, IP Mouse antibody
MRO-152LC Anti-HIV-1 gp120 Recombinant Antibody (G3-136) Neut, ELISA Mouse antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABW-064. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare