Recombinant Human Antibody (B13) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-HIV-1 gp120 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Unlike other CD4BS antibodies, antibody b13 showed substantial, though reduced, binding to the 109-428 variant. In addition, b13 was able to recognize an outer domain–only variant of gp120.
Figure 1 Effect of 17b on bNAb and non-bNAb binding to monomeric HXBc2 gp120 core by Sandwich ELISA assay.
Saturating amount of 17b was coated on ELISA plate and HXBc2 gp120 core was added to form complex with 17b, followed by the addition of biotinylated human MAbs (VRC01, PGV04, F105, b6, b13) and NHP MAbs (GE125, GE136 and GE140). Binding was detected with a streptavidin-HRP conjugate and the absorbance was read at 450 nm. OD, optical density.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
Figure 2 ELISA binding profiles of human CD4bs and non-CD4bs MAbs (left), and selected sera of Imm 3 and Imm 7 (right) from complex-GLA immunized rabbit group tested with CD4bs bNAb probes, RSC3/G367R and RSC3 Δ371I/P363N.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
Figure 3 The binding kinetics of CD4bs MAb b13 and representative rabbit MAbs 30-9 and 46-1 to HXBc2 core and RSC3 core.
Different concentrations of the core were shown.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
Figure 4 Surface plasmon resonance analysis
Surface plasmon resonance analysis of deglycosylated unlabeled, 15N and 15N/13C labeled binding to monoclonal antibodies b12 and b13 demonstrates that the expressed protein is correctly folded and biologically active.
Sastry, M., Xu, L., Georgiev, I. S., Bewley, C. A., Nabel, G. J., & Kwong, P. D. (2011). Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy. Journal of biomolecular NMR, 50(3), 197-207.
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CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-L012R | anti-env immunotoxin 907 (IgG)-RTA | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-193 | Recombinant Human Anti-HIV-1 gp41-M-MAT Antibody | Neut, FuncS | IgG |
PABZ-141 | Human Anti-ENV Recombinant Antibody (clone 2F5) | Neut | Human IgG |
PABZ-160 | Recombinant Human Anti-HIV-1 gp120 Antibody (PGT123) | FACS, Neut | IgG |
PABZ-161 | Recombinant Human Anti-HIV-1 gp120 Antibody (PGT122) | FACS, Neut | IgG |
PABZ-168 | Recombinant Human Anti-HIV-1 gp120 Antibody (PGT 135) | ELISA, Neut | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-193 | Recombinant Human Anti-HIV-1 gp41-M-MAT Antibody scFv Fragment | Neut, FuncS | scFv |
PFBZ-141 | Human Anti-ENV Recombinant Antibody (clone 2F5); Fab Fragment | Neut | Human Fab |
PFBW-167 | Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (537-10D) | ELISA, Neut | Fab |
PSBL-572 | Recombinant Human Anti-HPV Env Antibody scFv Fragment (mAb-94) | WB, IF, FuncS | scFv |
PFBC-210 | Recombinant Human Anti-HIV-1 gp120 Antibody Fab Fragment (3BNC60) | ELISA, Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-193 | Recombinant Human Anti-HIV-1 gp41-M-MAT Antibody Fab Fragment | Neut, FuncS | Fab |
PSBZ-141 | Human Anti-ENV Recombinant Antibody (clone 2F5); scFv Fragment | Neut | Human scFv |
PSBZ-168 | Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (PGT 135) | ELISA, Neut | scFv |
PSBZ-169 | Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (45-46M2) | ELISA, Neut | scFv |
PSBC-210 | Recombinant Human Anti-HIV-1 gp120 Antibody scFv Fragment (3BNC60) | ELISA, Neut | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-020 | Recombinant Anti-HIV ENV VHH Single Domain Antibody (JM4) | ELISA, SPR, Neut, FuncS | Llama VHH |
PNBL-021 | Recombinant Anti-HIV-1 ENV VHH Single Domain Antibody (2H10) | Neut, WB, ELISA, FuncS | Llama VHH |
PNBL-059 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody (D7) | ELISA, Inhib | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-CN0617 | PE-H-2Dd/HIV Env (IPGRAFYA) MHC Tetramer | FCM, IHC | |
MHC-CN0618 | APC-H-2Dd/HIV Env (IPGRAFYA) MHC Tetramer | FCM, IHC | |
MHC-CN0619 | FITC-H-2Dd/HIV Env (IPGRAFYA) MHC Tetramer | FCM, IHC | |
MHC-CN0620 | H-2Dd/HIV Env (IPGRAFYA) MHC Tetramer | FCM, IHC | |
MHC-CN0621 | PE-A*02:01/HIV Env (NVWATHACV) MHC Tetramer | FCM, IHC |
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