Recombinant Human Antibody (F105) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti- HIV-1 gp120 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- HIV-1 gp120 proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. F105 is a neutralizing, CD4–binding site (CD4BS) antibody, binding to gp120 occurred primarily through heavy-chain interactions, which were similar in many respects to those of CD4. F105 recognized conformational shifts in the position of the V1/V2 stem, resulting in a V1/V2 orientation poorly compatible with the functional viral spike.
Figure 1 Efficient cleavage of 4-2.J41 Env on the cell surface.
(C) Western blot analysis of immunoprecipitated 4-2.J41 Env protein from plasmamembrane fraction. Proteins from the plasma membrane fraction of Env transfected 293T cells were immunoprecipitated with VRC01, PGT151 and F105 antibodies and analyzed by Western blot using HIVIG as probe. M = molecular weight marker.
Boliar, S., Das, S., Bansal, M., Shukla, B. N., Patil, S., Shrivastava, T., ... & Chakrabarti, B. K. (2015). An efficiently cleaved HIV-1 clade C Env selectively binds to neutralizing antibodies. PloS one, 10(3), e0122443.
Figure 2 ELISA binding of selected monoclonal antibodies to soluble JR-FL gp140-foldon glycoprotein.
Selected control gp120-directed antibodies binding to the JR-FL gp140-foldon trimers.
Chakrabarti, B. K., Pancera, M., Phogat, S., O'Dell, S., McKee, K., Guenaga, J., ... & Wyatt, R. T. (2011). HIV type 1 Env precursor cleavage state affects recognition by both neutralizing and nonneutralizing gp41 antibodies. AIDS research and human retroviruses, 27(8), 877-887.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-380 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC06) | FC, Neut, FuncS | Human IgG |
PABW-060 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 58.2) | Neut | Mouse IgG1 |
PABC-061 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 83.1) | Neut | Mouse IgG1 |
PABZ-061 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 0.5β) | Neut | Mouse IgG |
PABC-062 | Recombinant Human Anti-HIV-1 gp120 Antibody (2G12) | Neut, ELISA | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-3386CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC26.11 (CAP256-VRC26.11)); Fab Fragment | ELISA, Neut | Human Fab |
MRO-3462CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9-PG16-RSH (PG9-16-RSH, PG9-16, PG916)); Fab Fragment | Neut | Human Fab |
MRO-3463CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone CD4-218.3-E51); Fab Fragment | ELISA | Human Fab |
MRO-3464CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone A16); Fab Fragment | Neut | Human Fab |
MRO-3465CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 5E8); Fab Fragment | ELISA, FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0057WJ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone L69); scFv Fragment | ELISA, Neut | Human scFv |
FAMAB-0058WJ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone P20); scFv Fragment | ELISA, Neut | Human scFv |
HPAB-0713-YJ-S(P) | Monkey Anti-gp120 Recombinant Antibody (GE331); scFv Fragment | ELISA, Neut | Monkey scFv |
HPAB-0714-YJ-S(P) | Monkey Anti-gp120 Recombinant Antibody (GE443); scFv Fragment | ELISA | Monkey scFv |
HPAB-0716-YJ-S(P) | Monkey Anti-gp120 Recombinant Antibody (2S1H3); scFv Fragment | ELISA, IHC | Monkey scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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