Rabbit Anti-HIV-1 gp120 C4 region Recombinant Antibody (clone R53) (CAT#: PABL-174)
Recombinant Rabbit Antibody (R53) is capable of binding to HIV-1 gp120 C4 region, expressed in mammalian cells.
Specific Inquiry
Figure 1 Competition assay of R53 with Ig-CD4. The soluble CD4 (sCD4) and anti-V3 mAb R56 were used as positive and negative controls, respectively. Results shown represent the mean levels of two independent experiments and error bars indicate the standard deviations.
Pan, R., Chen, Y., Vaine, M., Hu, G., Wang, S., Lu, S., & Kong, X. P. (2015). Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding.Emerging microbes & infections, 4(7), e44.
Figure 2 R53 bound nine gp120s from clades A, B, C, D, and AE, as revealed by ELISA.
Pan, R., Chen, Y., Vaine, M., Hu, G., Wang, S., Lu, S., & Kong, X. P. (2015). Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding.Emerging microbes & infections, 4(7), e44.
Figure 3 Western blot analysis showed that five selected gp120 proteins from different clades were recognized by R53.
Pan, R., Chen, Y., Vaine, M., Hu, G., Wang, S., Lu, S., & Kong, X. P. (2015). Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding.Emerging microbes & infections, 4(7), e44.
Figure 4 Binding kinetics of R53 and gp120s.
The binding kinetics of R53 to 92UG037 (clade A), JR-FL (clade B), 93MW965 (clade C), 92UG021 (clade D), and AE consensus (clade AE) were measured using ForteBio at different concentrations of the ligand. The brown, red, blue, purple, green, and orange lines represent 300 nM, 100 nM, 33.3 nM, 11.1 nM, 3.7 nM, and 1.23 nM of gp120 proteins, respectively.
Pan, R., Chen, Y., Vaine, M., Hu, G., Wang, S., Lu, S., & Kong, X. P. (2015). Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding.Emerging microbes & infections, 4(7), e44.
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- HIV-1 gp120 C4 region
- Species Reactivity
- HIV-1
- Clone
- R53
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA and WB. For details, refer to published data.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-160 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105); scFv Fragment | FC, Neut | Human scFv |
| PSBW-178 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone b12); scFv Fragment | Neut, ELISA | Human scFv |
| MRO-3081CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH276); Fab Fragment | Neut | Human Fab |
| MRO-3082CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH374); Fab Fragment | Neut | Human Fab |
| MRO-3083CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH375); Fab Fragment | Neut | Human Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
| MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
| MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
| MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
| MRO-032LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91H9) | ELISA | Single domain antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
| MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
| MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
| MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
| MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
| MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
| MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
| MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
| MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
| MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
| MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3344CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC23b); scFv Fragment | Neut | Human scFv |
| MRO-3345CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC24); scFv Fragment | Neut | Human scFv |
| MRO-3346CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC26.02 (CAP256-VRC26.02)); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-3347CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC26.04 (CAP256-VRC26.04)); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-3348CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 4210); scFv Fragment | Neut | Human scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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