Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone mAb 2158) (CAT#: PABL-175)

Figure 1 Titration of gp120BaL coated onto beads. Ten serial 3-fold dilutions of recombinant gp120BaL were used for coating the beads to test phagocytosis mediated by MAb 2158 (anti-V2) and negative-control MAb 1418 (anti-parvovirus B19). A suboptimal dose of 1.7 μg/ml of gp120 was used for coating the beads in the study.

Musich, T., Li, L., Liu, L., Zolla-Pazner, S., Robert-Guroff, M., & Gorny, M. K. (2017). Monoclonal Antibodies to V2, V3, the CD4-binding site and gp41 HIV-1 Mediate Phagocytosis in a Dose-dependent Manner. Journal of virology, JVI-02325.

Figure 2 Specific monoclonal antibodies inhibit gp120 binding at protein or integrin level.

CD4+ cells (left) and CD8+ cells (right) were incubated with biotinylated gp120 from clade A either without antibody pretreatment, red line (control) or after pretreatment with 2 mg of antiintegrin a4 mAb, HP2/1, (blue line), anti-V2 mAb 697D (orange line) or anti-V2 mAb 2158 (aqua). Binding was detected with NeutrAvadin PE and analyzed by FACS. The binding of clade A gp120 (red line) is inhibited by the addition of 2 mg anti-a4 mAb (blue line), anti-V2 mAb 697D (orange line) and anti-V2 mAb 2158 (aqua line). Green histogram is NeutrAvidin control, LFI = Log Fluorescence Intensity.

Tassaneetrithep, B., Tivon, D., Swetnam, J., Karasavvas, N., Michael, N. L., Kim, J. H.,... & Cardozo, T. (2014). Cryptic determinant of α4β7 binding in the V2 loop of HIV-1 gp120.PloS one, 9(9), e108446.

Figure 1 Anti-HIV-1 gp120 Antibody (PABL-175) in WB

Western blot analysis of PABL-175 was performed with HIV-1 gp120 Protein.

Figure 2 Anti-HIV-1 gp120 Antibody (PABL-175) in ELISA

ELISA analysis of PABL-175 was performed by coating with HIV-1 gp120 Protein.

Figure 3 Anti-HIV-1 gp120 Antibody (PABL-175) in Dot Blot

Dot Blot analysis of PABL-175 was performed by coating with HIV-1 gp120 Protein.