Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone mAb 2158)
CAT#: PABL-175
Recombinant Human Antibody (mAb 2158) is capable of binding to HIV-1 gp120 V2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 V2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp120 V2 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Tested Data
Figure 1 Titration of gp120BaL coated onto beads. Ten serial 3-fold dilutions of recombinant gp120BaL were used for coating the beads to test phagocytosis mediated by MAb 2158 (anti-V2) and negative-control MAb 1418 (anti-parvovirus B19). A suboptimal dose of 1.7 μg/ml of gp120 was used for coating the beads in the study.
Musich, T., Li, L., Liu, L., Zolla-Pazner, S., Robert-Guroff, M., & Gorny, M. K. (2017). Monoclonal Antibodies to V2, V3, the CD4-binding site and gp41 HIV-1 Mediate Phagocytosis in a Dose-dependent Manner. Journal of virology, JVI-02325.
Figure 2 Specific monoclonal antibodies inhibit gp120 binding at protein or integrin level.
CD4+ cells (left) and CD8+ cells (right) were incubated with biotinylated gp120 from clade A either without antibody pretreatment, red line (control) or after pretreatment with 2 mg of antiintegrin a4 mAb, HP2/1, (blue line), anti-V2 mAb 697D (orange line) or anti-V2 mAb 2158 (aqua). Binding was detected with NeutrAvadin PE and analyzed by FACS. The binding of clade A gp120 (red line) is inhibited by the addition of 2 mg anti-a4 mAb (blue line), anti-V2 mAb 697D (orange line) and anti-V2 mAb 2158 (aqua line). Green histogram is NeutrAvidin control, LFI = Log Fluorescence Intensity.
Tassaneetrithep, B., Tivon, D., Swetnam, J., Karasavvas, N., Michael, N. L., Kim, J. H.,... & Cardozo, T. (2014). Cryptic determinant of α4β7 binding in the V2 loop of HIV-1 gp120.PloS one, 9(9), e108446.
❮
❯
WB
Figure 1 Anti-HIV-1 gp120 Antibody (PABL-175) in WB
Western blot analysis of PABL-175 was performed with HIV-1 gp120 Protein.
ELISA
Figure 2 Anti-HIV-1 gp120 Antibody (PABL-175) in ELISA
ELISA analysis of PABL-175 was performed by coating with HIV-1 gp120 Protein.
DB
Figure 3 Anti-HIV-1 gp120 Antibody (PABL-175) in Dot Blot
Dot Blot analysis of PABL-175 was performed by coating with HIV-1 gp120 Protein.
❮
❯
Specifications
- Immunogen
- Human immunodeficiency virus 1 gp160; envelope glycoprotein
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Tested positive against HIV ENV
- Species Reactivity
- HIV
- Clone
- mAb 2158
- Applications
- ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for FC. For details, refer to published data.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABL-175. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone mAb 2158"
- CAT
- Product Name
See other products for "HIV-1 gp120 V2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3651CQ | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone L17) | ELISA | Human IgG |
| MRO-3652CQ | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 2297) | ELISA | Human IgG |
| MRO-3653CQ | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1361) | ELISA | Human IgG1, κ |
| MRO-3654CQ | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1357) | ELISA | Human IgG1, κ |
| MRO-3655CQ | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1393A) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3651CQ-S(P) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone L17); scFv Fragment | ELISA | Human scFv |
| MRO-3652CQ-S(P) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 2297); scFv Fragment | ELISA | Human scFv |
| MRO-3653CQ-S(P) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1361); scFv Fragment | ELISA | Human scFv |
| MRO-3654CQ-S(P) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1357); scFv Fragment | ELISA | Human scFv |
| PSBC-480 | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone RE505-22); scFv Fragment | ELISA, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3651CQ-F(E) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone L17); Fab Fragment | ELISA | Human Fab |
| MRO-3652CQ-F(E) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 2297); Fab Fragment | ELISA | Human Fab |
| MRO-3653CQ-F(E) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1361); Fab Fragment | ELISA | Human Fab |
| MRO-3654CQ-F(E) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1357); Fab Fragment | ELISA | Human Fab |
| MRO-3655CQ-F(E) | Human Anti-HIV-1 gp120 V2 Recombinant Antibody (clone 1393A); Fab Fragment | ELISA | Human Fab |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-3.png)
Application: ELISA, FC, Block, SPR
Application: WB, IF, FuncS
Application: Neut, Inhib, FC, ELISA
Application: ELISA, IHC, FC, WB, IF, IA
Application: ELISA
Application: ELISA, FC, Inhib
-4.jpg)
Application: FC
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
