Human Anti-HIV-1 gp41 Recombinant Antibody (clone D5) (CAT#: PABW-068)

Figure 1 HNP-1 synergizes with neutralizing antibodies to gp41 and with HIV serum in inhibiting HIV-1 fusion.

HXB2 pseudoviruses were pre-bound to human PBMCs in the cold, and fusion was initiated in the absence or in the presence of indicated antibodies by shifting to 37 u C. Data are normalized to the extent of fusion (measured by the BlaM assay) in the absence of defensin or antibodies (control). Addition of 24 m M HNP-1 in HBSS with 10% human serum did not affect HXB2 fusion. The following concentrations of antibodies were used: D5 (25 m g/ml), bF-3674 (12 m g/ml), 2F5 (2.5 m g/ml), PG9 (20 m g/ml). HIV-1 neutralizing serum was diluted 1:15 prior to adding to cells. Experiments were performed either in the absence (black bars) or in the presence (red bars) of 24 m M HNP-1 in 10% human serum. Experiment with 3 m M C52L were used as a negative control.

Demirkhanyan, L., Marin, M., Lu, W., & Melikyan, G. B. (2013). Sub-inhibitory concentrations of human α-defensin potentiate neutralizing antibodies against HIV-1 gp41 pre-hairpin intermediates in the presence of serum. PLoS pathogens, 9(6), e1003431.

Figure 2 Antiviral activity of D5.

(A) D5 scFv and IgG1 inhibit HIV entry in the HIVRP assay. The bluegreen fluorescence ratio is proportional to virus entry. X5 scFv is shown as a control. (B) D5 scFv and IgG1 inhibit HIV infectivity in a single-cycle infection assay. X5 scFv is shown as a control.

Miller, M. D., Geleziunas, R., Bianchi, E., Lennard, S., Hrin, R., Zhang, H., ... & Mattu, M. (2005). A human monoclonal antibody neutralizes diverse HIV-1 isolates by binding a critical gp41 epitope. Proceedings of the National Academy of Sciences, 102(41), 14759-14764.

Figure 2 Competition ELISA using IgGs D5 and 8K8, and Fab DN9 against their biotinylated (Bio) counterparts for recognition of immobilized 5-Helix.

Bio-8K8 IgG (left panel), Bio-D5 IgG (middle panel), and Bio-DN9 Fab (right panel) at concentrations previously determined to generate non-saturating binding signals were added to wells coated with immobilized 5-Helix that were pretreated with various concentrations of competing mAb. Results are plotted as a percentage of the maximal ELISA binding signal of the Bio-mAb in the absence of competitor.

Nelson, J. D., Kinkead, H., Brunel, F. M., Leaman, D., Jensen, R., Louis, J. M., ... & Dawson, P. E. (2008). Antibody elicited against the gp41 N-heptad repeat (NHR) coiled-coil can neutralize HIV-1 with modest potency but non-neutralizing antibodies also bind to NHR mimetics. Virology, 377(1), 170-183.