Mouse Anti-HIV-2 Recombinant Antibody (clone 7C8) (CAT#: PABX-104)

Figure 1 Western blot analysis of 7C8 and 3C4 binding to the different glycoproteins.

a) Recognition of gp125 (I) and gp125Δv1v2 (II) by 7C8 in nonreducing conditions. Oligomers recognized by 7C8 are indicated by arrows. b) 7C8 binding to gp125 (I) and gp125Δv1v2 (II) in reducing conditions. c) GN-purified gp125 (I) and gp125Δv1v2 (II) recognition by 3C4 in non-reducing conditions. Oligomers are indicated by arrows.

Sourial, S., Nilsson, C., Warnmark, A., Achour, A., & Harris, R. A. (2006). Deletion of the V1/V2 region does not increase the accessibility of the V3 region of recombinant gp125. Current HIV research, 4(2), 229-237.

Figure 2 SPR analysis of 7C8 and CD4 binding to the different glycoproteins.

a) 7C8 binding to equimolar amounts of gp125 (bold) and gp125Δv1v2 (dashed) immobilized onto individual surfaces of a CM5 chip, b) CD4 binding to equimolar amounts of HIV-1 gp120 (light), gp125Δv1v2 (bold),or gp125 (dashed)CM5 immobilized on CM5 chip surfaces.

Sourial, S., Nilsson, C., Warnmark, A., Achour, A., & Harris, R. A. (2006). Deletion of the V1/V2 region does not increase the accessibility of the V3 region of recombinant gp125. Current HIV research, 4(2), 229-237.

Figure 3 Overall three-dimensional structure of the HIV-2 neutralizing Fab fragment 7C8.

A) Overall view of the crystal structure of the 7C8 Fab fragment. The light and heavy chains are displayed in light and dark grey, respectively. The six CDR loops are in dark blue (CDRL1), light blue (CDRL2), blue (CDRL3), brown (CDRH1), red (CDRH2) and pink (CDRH3). A black dashed line indicates the disordered loop within the first constant region of the CH1 domain. B) Stick representation of the stretch of residues 93 to 100C corresponding to the unusually long third complementary determining region of the heavy chain (CDRH3) with the electron density carved around the model at 1.2 s. C) Ribbon representation of the two monomers found in the assymmetric unit indicating the large variation in elbow angle.

Uchtenhagen, H., Friemann, R., Raszewski, G., Spetz, A. L., Nilsson, L., & Achour, A. (2011). Crystal structure of the HIV-2 neutralizing Fab fragment 7C8 with high specificity to the V3 region of gp125. PloS one, 6(4), e18767.