Human Anti-HIV Env Recombinant Antibody (clone 10-1074)
CAT#: PABL-508
Recombinant Human Antibody (10-1074) is capable of binding to HIV env, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV env mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV env proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody exhibits unusual potent neutralization, including broad reactivity against newly transmitted viruses.
Published Data
Tested Data
FC
Figure 1 Antibody binding at the surface of cells infected with two HIV-1 reference strains.
CEM-NKR cells infected with HIV-1 (NLAD8) were incubated with 15 μg/ml of anti-Env monoclonal antibodies. The levels of antibody bound on infected (Gag-high) and bystander (Gag-low and Gag-negative) cells were then evaluated by flow cytometry.
Bruel, T., Guivel-Benhassine, F., Lorin, V., Lortat-Jacob, H., Baleux, F., Bourdic, K., ... & Schwartz, O. (2017). Lack of ADCC breadth of human nonneutralizing anti-HIV-1 antibodies. Journal of virology, 91(8), e02440-16.
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SDS-PAGE
Figure 1 Human Anti-HIV Env Recombinant Antibody (PABL-508) in SDS-PAGE
SDS-PAGE analysis of PABL-508 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, the different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABL-508 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Human Anti-HIV Env Recombinant Antibody (PABL-508) in SEC-HPLC
The purity of PABL-508 was 99.4989% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Human Anti-HIV Env Recombinant Antibody (PABL-508) in WB
Western blot analysis of PABL-508 was performed by loading recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-508 and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Human Anti-HIV Env Recombinant Antibody (PABL-508) in DB
Dot Blot analysis of PABL-508 was performed by coating with recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein (His tag).
PABL-508 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Human Anti-HIV Env Recombinant Antibody (PABL-508) in ELISA
ELISA analysis of PABL-508 was performed by coating with recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag). Then blocked with BSA and incubated with PABL-508. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- HIV gp160; envelope glycoprotein
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Tested positive against HIV-1 Env
- Species Reactivity
- HIV
- Clone
- 10-1074
- Applications
- FC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry and Function Assay.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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