Anti-Human HMG1 Recombinant Antibody scFv Fragment (TAB-100CT-S(P))

Name: Anti-Human HMG1 Recombinant Antibody scFv Fragment (TAB-100CT-S(P))
SKU: TAB-100CT-S(P)
Rating: 5 (1 reviews)

CAT#: TAB-100CT-S(P)

Recombinant monoclonal antibody to HMG1. This antibody intended for the prophylaxis and treatment of HMGB1-related diseases .

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg

Subcellular Location Figure 3 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg

Subcellular Location Figure 4 IHC staining of human esophagus

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg

Normal Tissue Figure 8 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

❮

❯

Specifications

Immunogen
17-mer peptide P1 (KGKPDAAKKGVVKAEKS)

Host Species
Mouse

Derivation
Hybridoma cell line

Specificity
Human

Species Reactivity
Human

Applications
WB, IF, FC, FuncS, Transmigration assay

Related Disease
HMGB1-related diseases

Applications

Application Notes
Anti-HMGB-1 Ab (DPH1.1 Ab) characterization and specificity testing by Western blot
Western blots of the recombinant BoxA domain of HMGB1 (negative control) incubated with either the anti-HMGB-1 mAb DPH1.1 or a control anti-BoxA mAb control were performed. Briefly, 500 ng or 100 ng of recombinant HMGB1 and the (negative control) recombinant BoxA domain of HMGB1 were separated by gel electrophoresis and transferred onto membranes.
Immunofluorescence
DPH1.1 mAb, anti-BoxA mAb and goat anti- mouse lgG1 Ab were applied at 1μg/ml dilution. Immunofluorescence was performed on mouse embryonic fibroblasts (MEFs) derived from either wild type mice or HmgbV¹ mice. DPH1.1 mAb and AlexaFluor 633-labelled goat anti-mouse lgG1 Ab were applied at a 50 μg/ml dilution.
Transmigration assay
Recombinant HMGB1 was added to the lower chamber at the concentration of 30 ng/ml. Increasing concentrations of DPH1. 1 mAb (or of an irrelevant isotype-matched mAb) were added to fifty thousand 3T3 cells. Cell migration was assessed by a modified Boyden chamber assay. Boyden chambers were incubated at 37 °C in 5% CO₂ for 3 hrs. Cells remaining on the upper section of the filters were removed mechanically. Cells that migrated to the lower section of the filters were fixed with ethanol, stained with Giemsa, and counted in 10 random fields/filter.
In vivo experiments
Inflammatory cell recruitment Recruitment of inflammatory cells can be amplified by removal of Kupffer cells (KCs) from the liver, which can be obtained by treatment of the mice with clodronate (Clo-L). In order to amplify the effect of HMGB1, Kupffer cells (KCs) of HBV transgenic mice were removed, and injected with CD8 T cells from mice immunized against HBV (this causes acute hepatitis in the mice).

Target

Alternative Names
HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; anti-HMGB1

Gene ID
3146

UniProt ID
A0A024RDR0

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for TAB-100CT-S(P). Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-100CT-S(P).pdf

MSDS

MSDS - TAB-100CT-S(P).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "HMGB1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
DrMAB-956	Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4)	ELISA, WB	Human IgG
MOB-0839CT	Recombinant Mouse anti-Human HMGB1 Monoclonal antibody (19N10B7)	ELISA, FC, ICC, IF, IHC-P, WB
MRO-1938-CN	Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN)	WB, IF, IHC, FC	Rabbit IgG
MRO-1939-CN	Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN)	WB, IF, IHC, FC	Rabbit IgG
VS3-WK1655	Recombinant Mouse Anti-HMGB1 Detection Antibody (clone 1G1)	ELISA	Mouse IgG2b

CAT	Product Name	Application	Type
TAB-0315CL-S(P)	Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0315CL-S(P))	ELISA, Inhib, FuncS, WB	Mouse scFv
TAB-0316CL-S(P)	Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0316CL-S(P))	ELISA, Inhib, FuncS, WB	Mouse scFv
TAB-0317CL-S(P)	Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0317CL-S(P))	ELISA, Inhib, FuncS, WB	Mouse scFv
TAB-0318CL-S(P)	Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-0318CL-S(P))	ELISA, Inhib, FuncS, WB	Mouse scFv
TAB-100CT	Anti-Human HMG1 Recombinant Antibody (TAB-100CT)	IF, FC

CAT	Product Name	Application	Type
TAB-099CT	Human Anti-HMGB1 Recombinant Antibody (TAB-099CT)	IF, ELISA, IHC	Human IgG1, λ
TAB-099CT-S(P)	Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P))	ELISA, FC, Block	Human scFv
TAB-099CT-F(E)	Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E))	ELISA, FC, Block	Humanized Fab

CAT	Product Name	Application	Type
TAB-101CT	Anti-Human HMG1 Recombinant Antibody	ELISA, BL	Human antibody
TAB-101CT-S(P)	Anti-Human HMG1 Recombinant Antibody scFv Fragment	ELISA, BL	Human antibody
TAB-101CT-F(E)	Anti-Human HMG1 Recombinant Antibody Fab Fragment	ELISA, BL	Human antibody

CAT	Product Name	Application	Type
MOR-1633	Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB)	WB, IHC-P, FC	Rabbit IgG
MOR-0049-FY	Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020)	ICC, IHC-P, WB	Rabbit IgG

CAT	Product Name	Application	Type
MHC-LC2082	PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer	FCM
MHC-LC2083	APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer	FCM

CAT	Product Name	Application	Type
HPAB-0215-FY-S(P)	Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment	ELISA	Mouse scFv
HPAB-N0137-YC-S(P)	Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P))	ELISA	Mouse scFv
HPAB-0101-WJ-S(P)	Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P))	ELISA, WB	Humanized scFv

CAT	Product Name	Application	Type
HPAB-0215-FY-F(E)	Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment	ELISA	Mouse Fab
HPAB-N0137-YC-F(E)	Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E))	ELISA	Mouse Fab
HPAB-0101-WJ-F(E)	Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E))	ELISA, WB	Humanized Fab

CAT	Product Name	Application	Type
VS3-WK1654	Recombinant Mouse Anti-HMGB1 Capture Antibody (clone 1B5)	ELISA	Mouse IgM

CAT	Product Name	Application	Type
VS-0724-YC699	AbPlus™ Anti-HMGB1 Magnetic Beads (VS-0724-YC699)	IP, Protein Purification

CAT	Product Name	Application
VS-0325-XY1033	Anti-HMGB1 Immunohistochemistry Kit	IHC
VS-0525-XY3204	Anti-Mouse HMGB1 Immunohistochemistry Kit	IHC
VS-0525-XY3206	Anti-Human HMGB1 Immunohistochemistry Kit	IHC
VS-0525-XY3205	Anti-Rat HMGB1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS13-YC535	CytoStream™ Rabbit Anti-HMGB1 Recombinant Antibody (VS13-YC535)	WB, ICC, IF, IHC-P, FC	Rabbit IgG

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-CD248 Recombinant Antibody (TAB-773) in HPLC

Anti-CD248 Recombinant Antibody (Ontuxizumab) (CAT# TAB-773)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Figure 3 Anti-LY6E Recombinant Antibody (MOB-636) in ELISA

Recombinant Anti-human LY6E Antibody (CAT# MOB-636)
Application: WB, FC, FuncS

Figure 1 Anti-P. aeruginosa psl Antibody in SDS-PAGE

Recombinant Anti-P. aeruginosa psl Antibody (CAT# MOB-736)
Application: WB, IP, FuncS

Figure 2 Anti-Human CD200 Recombinant Antibody (TAB-210) in HPLC

Anti-Human CD200 Recombinant Antibody (TAB-210) (CAT# TAB-210)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 2 Anti-SDC1 Antibody (TAB-197) in ELISA

Chimeric (Mouse/Human) Anti-SDC1 Recombinant Antibody (TAB-197) (CAT# TAB-197)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB

Anti-Human IL4 Recombinant Antibody (TAB-221) (CAT# TAB-221)
Application: WB, ELISA, FC, IP, FuncS, IF, Neut

Figure 2 Anti-Human IL6R Antibody (TAB-026) in WB

Anti-Human IL6R Recombinant Antibody (TAB-026) (CAT# TAB-026)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 5 Anti-Human EGFR Recombinant Antibody (Modotuximab) (TAB-H49) in DB

Anti-Human EGFR Recombinant Antibody (Modotuximab) (CAT# TAB-H49)
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC

Figure 2 Anti-Human MET Recombinant Antibody (TAB-521CL) in WB

Anti-Human MET Recombinant Antibody (TAB-521CL) (CAT# TAB-521CL)
Application: WB, FuncS

Figure 2 Anti-Human LAG3 Recombinant Antibody (TAB-0367CL) in ELISA

Anti-Human LAG3 Recombinant Antibody (TSR-033) (CAT# TAB-0367CL)
Application: FC, FuncS, Block

Figure 2 Human Anti-CD3 Recombinant Antibody (TAB-0416CL-S(P)) in WB

Mouse Anti-CD3 Recombinant Antibody scFv Fragment (TAB-0416CL-S(P)) (CAT# TAB-0416CL-S(P))
Application: IB, ELISA, FC, FuncS

Figure 3 Anti-Human CD34 Recombinant Antibody (TAB-150LC) in ELISA

Human Anti-CD34 Recombinant Antibody (TAB-150LC) (CAT# TAB-150LC)
Application: ELISA

Figure 2 Anti-CLDN4 Antibody (TAB-501LC) in HPLC

Anti-Human CLDN4 Recombinant Antibody (KM3900) (CAT# TAB-501LC)
Application: WB, IP, FC

Figure 2 Anti-Human RNF43 Recombinant Antibody scFv Fragment (TAB-328CT-S(P)) in HPLC

Humanized Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-328CT-S(P)) (CAT# TAB-328CT-S(P))
Application: WB, ELISA, FC, IHC, IP

Figure 2 Recombinant Human Anti-EGFR Antibody (PABX-052) in HPLC

Recombinant Human Anti-EGFR Antibody (PABX-052) (CAT# PABX-052)
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop

Figure 4 Recombinant Mouse Anti-Integrin alpha 4 Antibody (ACT-1), Fc silenced (PABX-122-FcS) in ELISA

Recombinant Mouse Anti-Integrin alpha 4 Antibody (ACT-1) (CAT# PABX-122)
Application: Neut, FuncS

Figure 1 Recombinant Human Anti-HHV-5 strain AD169 gB Antibody (EPAF-0438CQ) in SDS-PAGE

Recombinant Human Anti-HHV-5 strain AD169 gB Antibody (ITC88) (CAT# EPAF-0438CQ)
Application: ELISA, Neut

Figure 14 Human Anti-IAV HA Antibody (PABC-393) in DB 4

Recombinant Human Anti-IAV HA Antibody (PABC-393) (CAT# PABC-393)
Application: Neut, FuncS, ELISA

Figure 3 Human Anti-IL21R Recombinant Antibody (FAMAB-1203CQ) in DB

Human Anti-IL21R Recombinant Antibody (clone ATR-107) (CAT# FAMAB-1203CQ)
Application: Inhib, FC, WB

Figure 5 Human Anti-EBV gB Recombinant Antibody (Clone AMMO2) (PABS-0037) in DB

Human Anti-EBV gB Recombinant Antibody (Clone AMMO2) (CAT# PABS-0037)
Application: ELISA, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.