Recombinant Mouse Anti-HPV-16 E2 Antibody (ED23) (CAT#: EPAF-0620LC)

1. Q 1: What is the primary application of the recombinant mouse anti-HPV-16 E2 antibody in research?

   A: The recombinant mouse anti-HPV-16 E2 antibody is primarily used to study the E2 protein of HPV-16, which plays a crucial role in regulating viral DNA replication. This antibody is valuable in investigating the mechanisms of HPV-16 in cervical cancer and other HPV-associated diseases.

2. Q 2: What are the optimal assay conditions for using the recombinant mouse anti-HPV-16 E2 antibody in Western blotting?

   A: For Western blotting, the recommended starting dilution for the recombinant mouse anti-HPV-16 E2 antibody is 1:1000. Researchers should optimize this dilution based on their specific experimental setup and sample type to ensure accurate results.

3. Q 3: Can the recombinant mouse anti-HPV-16 E2 antibody be used for immunofluorescence?

   A: Yes, the recombinant mouse anti-HPV-16 E2 antibody can be used in immunofluorescence assays. It is effective in detecting the E2 protein within cells, providing insights into the subcellular localization and dynamics of HPV-16 E2.

4. Q 4: What type of sample preparation is required for using the recombinant mouse anti-HPV-16 E2 antibody in immunohistochemistry?

   A: For immunohistochemistry, tissue samples should be properly fixed, typically in formalin, and then embedded in paraffin. Sections should be deparaffinized and rehydrated before applying the antibody. Antigen retrieval may be necessary to expose epitopes and enhance staining.

5. Q 5: What is the expected molecular weight of the HPV-16 E2 protein detected by the recombinant mouse anti-HPV-16 E2 antibody?

   A: The HPV-16 E2 protein detected by this antibody has an approximate molecular weight of 42 kDa. This allows for its identification and analysis in various assays, including Western blotting.

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