Recombinant Mouse Anti-HPV16 Antibody (H16.V5)
CAT#: PABL-200
Recombinant Mouse Antibody (H16.V5) is capable of binding to HPV16, expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Figure 1 Binding of 16 and 52 specific MAbs to VLPs with mutations of the BC loop. VLPs 16:wt (▪), 16:BC (□), 52:wt (•), and 52:BC (○) were titrated across a plate and reacted with CAMVIR-1, H16.V5, H16.E70, H52.B9, and H52.D12 in an ELISA.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 2 Binding of MAbs to HPV-16/31 hybrid VLPs.
MAbs were tested by ELISA for reactivity to HPV-16 and HPV-31 wild-type VLPs and mutated VLPs that are indicated on the x-Axis. The y-axis are normalized optical density values (see text) for H16.V5 (solid), H16.E70 (striped), H16.U4 (cross-hatched), and H31.A6 (opposite striped) binding.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 3 Identification of residues on the FG loop important for MAb binding.
The sequence of HPV-16 and HPV-31 L1 are compared (A). Residues that are predicted to be>45% surface accessible are indicated with arrows and bold type is used to indicate nonconserved residues. CAMVIR-1, H16.V5, H16.E70, and H16.U4 were tested for binding to VLPs that were serially diluted prior to testing. The normalized binding values are presented (B), with each MAb represented by bars.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 4 EF loop is necessary and sufficient for H31.A6 binding.
VLPs were serially diluted across microtiter plates and ELISAs performed with CAMVIR-1, H16.V5 or H31.A6 as indicated. The VLPs were HPV-16:wt (▪), HPV-31:wt (•), 16:EF (□), and 31:EF (○).
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 5 Inhibition of 26D1 (a) and H16.V5 (b) binding to HPV16 VLP by pre-binding of heparin.
Insets: dose response curves showing the inhibition of 26D1 or H16.V5 binding by the soluble receptor molecule heparin in solution. The median inhibitory concentration (IC50) values for 26D1 (40 mg/ml) and H16.V5 (100 mg/ml) were approximated by linear regression fitting the data (inhibition rate vs. heparin concentration) from multiple runs (n = 6).
Xia, L., Xian, Y., Wang, D., Chen, Y., Huang, X., Bi, X.,... & An, Z. (2016). A human monoclonal antibody against HPV16 recognizes an immunodominant and neutralizing epitope partially overlapping with that of H16. V5. Scientific reports, 6, 19042.
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Specifications
- Immunogen
- Human papillomavirus 16
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native HPV16 HPV16
- Species Reactivity
- HPV16
- Clone
- H16.V5
- Applications
- WB, ELISA, Neut, FuncS, Inhib
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA and Inhib. For details, refer to published data.
Target
- Alternative Names
- Human papillomavirus 16; HPV16
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Exceptional Sensitivity in Cervical Cancer Research
The recombinant mouse anti-HPV16 antibody from Creative Biolabs exhibits exceptional sensitivity, significantly enhancing detection accuracy in cervical cancer research. Its superior specificity outperforms other products, ensuring reliable and reproducible results.
Enhanced Outcomes in HPV Vaccine Development
The recombinant mouse anti-HPV16 antibody from Creative Biolabs significantly enhances research outcomes in HPV vaccine development. Its high affinity and specificity ensure precise detection, surpassing the capabilities of other similar antibodies.
Q&As
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What is the recommended protocol for using the recombinant mouse anti-HPV16 antibody in immunohistochemistry (IHC)?
A: The protocol for using the recombinant mouse anti-HPV16 antibody in IHC involves tissue preparation, antigen retrieval, blocking, and antibody incubation. It is crucial to follow a standardized protocol, including the use of appropriate controls, to ensure specific staining and minimal background.
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How should the recombinant mouse anti-HPV16 antibody be stored to maintain its stability and efficacy?
A: The recombinant mouse anti-HPV16 antibody should be stored at -20°C for long-term storage. For short-term use, storing at 2-8°C is recommended. Avoid repeated freeze-thaw cycles, as they can degrade the antibody's effectiveness. Proper storage conditions are essential to maintain the antibody's stability and binding capacity.
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Are there any known cross-reactivities of the recombinant mouse anti-HPV16 antibody with other HPV types?
A: The recombinant mouse anti-HPV16 antibody is designed to specifically target HPV16. However, it is crucial to verify its specificity through proper validation experiments. Cross-reactivity with other HPV types can be tested using control samples to ensure the antibody's specificity and reliability in detecting HPV16.
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How can the performance of the recombinant mouse anti-HPV16 antibody be validated in ELISA assays?
A: The performance of the recombinant mouse anti-HPV16 antibody in ELISA assays can be validated by testing its binding efficiency and specificity using HPV16 positive samples and appropriate controls. Standard curves should be generated to quantify the antigen-antibody interactions accurately. Proper validation ensures reliable and reproducible results in ELISA applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "Clone H16.V5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-200 | Recombinant Mouse Anti-HPV16 Antibody scFv Fragment (H16.V5) | WB, ELISA, Neut, FuncS | scFv |
| PSBL-573 | Recombinant Mouse Anti-HPV16 Antibody scFv Fragment (H16.1A) | Neut | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-200 | Recombinant Mouse Anti-HPV16 Antibody Fab Fragment (H16.V5) | WB, ELISA, Neut, FuncS | Fab |
| PFBL-573 | Recombinant Mouse Anti-HPV16 Antibody Fab Fragment (H16.1A) | Neut | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-578 | Recombinant Mouse Anti-HPV16 Antibody (PABL-578) | Neut | IgG |
| MRO-1868CQ | Recombinant Mouse Anti-HPV-16 Antibody (MRO-1868CQ) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-590LC-S(P) | Anti-HPV16 Recombinant Antibody scFv Fragment (4D11) | ELISA | Mouse antibody |
| MRO-591LC-S(P) | Anti-HPV16 Recombinant Antibody scFv Fragment (B9) | ELISA | Mouse antibody |
| MRO-590LC-F(E) | Anti-HPV16 Recombinant Antibody Fab Fragment (4D11) | ELISA | Mouse antibody |
| MRO-591LC-F(E) | Anti-HPV16 Recombinant Antibody Fab Fragment (B9) | ELISA | Mouse antibody |
| MRO-592LC-F(E) | Anti-HPV16 Recombinant Antibody Fab Fragment (11E3) | ELISA | Mouse antibody |
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