Recombinant Mouse Anti-HPV16 Antibody (H16.V5) (CAT#: PABL-200)

Recombinant Mouse Antibody (H16.V5) is capable of binding to HPV16, expressed in Chinese Hamster Ovary cells (CHO).


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  • Published Data
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Figure 1 Binding of 16 and 52 specific MAbs to VLPs with mutations of the BC loop. VLPs 16:wt (▪), 16:BC (□), 52:wt (•), and 52:BC (○) were titrated across a plate and reacted with CAMVIR-1, H16.V5, H16.E70, H52.B9, and H52.D12 in an ELISA.

Figure 1 Binding of 16 and 52 specific MAbs to VLPs with mutations of the BC loop. VLPs 16:wt (▪), 16:BC (□), 52:wt (•), and 52:BC (○) were titrated across a plate and reacted with CAMVIR-1, H16.V5, H16.E70, H52.B9, and H52.D12 in an ELISA.

Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.

Figure 2 Binding of MAbs to HPV-16/31 hybrid VLPs.

Figure 2 Binding of MAbs to HPV-16/31 hybrid VLPs.

MAbs were tested by ELISA for reactivity to HPV-16 and HPV-31 wild-type VLPs and mutated VLPs that are indicated on the x-Axis. The y-axis are normalized optical density values (see text) for H16.V5 (solid), H16.E70 (striped), H16.U4 (cross-hatched), and H31.A6 (opposite striped) binding.

Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.

Figure 3 Identification of residues on the FG loop important for MAb binding.

Figure 3 Identification of residues on the FG loop important for MAb binding.

The sequence of HPV-16 and HPV-31 L1 are compared (A). Residues that are predicted to be>45% surface accessible are indicated with arrows and bold type is used to indicate nonconserved residues. CAMVIR-1, H16.V5, H16.E70, and H16.U4 were tested for binding to VLPs that were serially diluted prior to testing. The normalized binding values are presented (B), with each MAb represented by bars.

Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.

Figure 4 EF loop is necessary and sufficient for H31.A6 binding.

Figure 4 EF loop is necessary and sufficient for H31.A6 binding.

VLPs were serially diluted across microtiter plates and ELISAs performed with CAMVIR-1, H16.V5 or H31.A6 as indicated. The VLPs were HPV-16:wt (▪), HPV-31:wt (•), 16:EF (□), and 31:EF (○).

Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.

Figure 5 Inhibition of 26D1 (a) and H16.V5 (b) binding to HPV16 VLP by pre-binding of heparin.

Figure 5 Inhibition of 26D1 (a) and H16.V5 (b) binding to HPV16 VLP by pre-binding of heparin.

Insets: dose response curves showing the inhibition of 26D1 or H16.V5 binding by the soluble receptor molecule heparin in solution. The median inhibitory concentration (IC50) values for 26D1 (40 mg/ml) and H16.V5 (100 mg/ml) were approximated by linear regression fitting the data (inhibition rate vs. heparin concentration) from multiple runs (n = 6).

Xia, L., Xian, Y., Wang, D., Chen, Y., Huang, X., Bi, X.,... & An, Z. (2016). A human monoclonal antibody against HPV16 recognizes an immunodominant and neutralizing epitope partially overlapping with that of H16. V5. Scientific reports, 6, 19042.


Specifications

  • Immunogen
  • Human papillomavirus 16
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native HPV16 HPV16
  • Species Reactivity
  • HPV16
  • Clone
  • H16.V5
  • Applications
  • WB, ELISA, Neut, FuncS, Inhib

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody was validated for ELISA and Inhib. For details, refer to published data.

Target

  • Alternative Names
  • Human papillomavirus 16; HPV16

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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