Recombinant Mouse Antibody (H16.V5) is capable of binding to HPV16, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Binding of 16 and 52 specific MAbs to VLPs with mutations of the BC loop. VLPs 16:wt (▪), 16:BC (□), 52:wt (•), and 52:BC (○) were titrated across a plate and reacted with CAMVIR-1, H16.V5, H16.E70, H52.B9, and H52.D12 in an ELISA.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 2 Binding of MAbs to HPV-16/31 hybrid VLPs.
MAbs were tested by ELISA for reactivity to HPV-16 and HPV-31 wild-type VLPs and mutated VLPs that are indicated on the x-Axis. The y-axis are normalized optical density values (see text) for H16.V5 (solid), H16.E70 (striped), H16.U4 (cross-hatched), and H31.A6 (opposite striped) binding.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 3 Identification of residues on the FG loop important for MAb binding.
The sequence of HPV-16 and HPV-31 L1 are compared (A). Residues that are predicted to be>45% surface accessible are indicated with arrows and bold type is used to indicate nonconserved residues. CAMVIR-1, H16.V5, H16.E70, and H16.U4 were tested for binding to VLPs that were serially diluted prior to testing. The normalized binding values are presented (B), with each MAb represented by bars.
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 4 EF loop is necessary and sufficient for H31.A6 binding.
VLPs were serially diluted across microtiter plates and ELISAs performed with CAMVIR-1, H16.V5 or H31.A6 as indicated. The VLPs were HPV-16:wt (▪), HPV-31:wt (•), 16:EF (□), and 31:EF (○).
Carter, J. J., Wipf, G. C., Benki, S. F., Christensen, N. D., & Galloway, D. A. (2003). Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. Journal of virology, 77(21), 11625-11632.
Figure 5 Inhibition of 26D1 (a) and H16.V5 (b) binding to HPV16 VLP by pre-binding of heparin.
Insets: dose response curves showing the inhibition of 26D1 or H16.V5 binding by the soluble receptor molecule heparin in solution. The median inhibitory concentration (IC50) values for 26D1 (40 mg/ml) and H16.V5 (100 mg/ml) were approximated by linear regression fitting the data (inhibition rate vs. heparin concentration) from multiple runs (n = 6).
Xia, L., Xian, Y., Wang, D., Chen, Y., Huang, X., Bi, X.,... & An, Z. (2016). A human monoclonal antibody against HPV16 recognizes an immunodominant and neutralizing epitope partially overlapping with that of H16. V5. Scientific reports, 6, 19042.
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CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-200 | Recombinant Mouse Anti-HPV16 Antibody scFv Fragment (H16.V5) | WB, ELISA, Neut, FuncS | scFv |
PSBL-573 | Recombinant Mouse Anti-HPV16 Antibody scFv Fragment (H16.1A) | Neut | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-200 | Recombinant Mouse Anti-HPV16 Antibody Fab Fragment (H16.V5) | WB, ELISA, Neut, FuncS | Fab |
PFBL-573 | Recombinant Mouse Anti-HPV16 Antibody Fab Fragment (H16.1A) | Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-578 | Recombinant Mouse Anti-HPV16 Antibody (H16.1A) | Neut | IgG |
MRO-1868CQ | Recombinant Mouse Anti-HPV-16 Antibody (M8011535) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-590LC | Anti-HPV16 Recombinant Antibody (4D11) | ELISA | Mouse antibody |
MRO-591LC | Anti-HPV16 Recombinant Antibody (B9) | ELISA | Mouse antibody |
MRO-590LC-S(P) | Anti-HPV16 Recombinant Antibody scFv Fragment (4D11) | ELISA | Mouse antibody |
MRO-591LC-S(P) | Anti-HPV16 Recombinant Antibody scFv Fragment (B9) | ELISA | Mouse antibody |
MRO-592LC-S(P) | Anti-HPV16 Recombinant Antibody scFv Fragment (11E3) | ELISA | Mouse antibody |
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