Anti-HPV16 E6 Recombinant Antibody (1B2.27) (CAT#: MRO-547LC)

1. Q 1: How can the anti-HPV16 E6 recombinant antibody be used in immunofluorescence?

   A: For immunofluorescence, fix and permeabilize the cells before blocking non-specific binding sites with a blocking buffer containing 5% BSA. Incubate the cells with the anti-HPV16 E6 recombinant antibody, followed by a fluorophore-conjugated secondary antibody. Visualize the staining using a fluorescence microscope.

2. Q 2: How should samples be prepared for Western blot analysis using anti-HPV16 E6 recombinant antibody?

   A: Prepare samples by lysing cells or tissues in an appropriate buffer, separate proteins by SDS-PAGE, and transfer them to a PVDF or nitrocellulose membrane. Block the membrane, incubate with the primary antibody, wash, and then incubate with a secondary antibody. Detect the signal using a chemiluminescent or colorimetric substrate.

3. Q 3: What are the recommended conditions for blocking non-specific binding when using anti-HPV16 E6 recombinant antibody in immunohistochemistry?

   A: Use a blocking buffer containing 5% BSA or normal serum to block non-specific binding sites. Incubate tissue sections with the blocking buffer for at least 30 minutes before applying the primary antibody. This helps reduce background staining and improves signal specificity.

4. Q 4: How can the anti-HPV16 E6 recombinant antibody be conjugated for use in different applications?

   A: The antibody can be conjugated to various labels such as fluorophores, biotin, or enzymes using conjugation kits. Follow the kit's instructions to mix the antibody with the labeling reagent, incubate, and purify the conjugated antibody to remove any unbound label.

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