Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0325CL)
CAT#: TAB-0325CL
This product is a recombinant mouse antibody that recognizes HS6ST2. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/2020_B5_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/69453_B_9_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/69453_B_8_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/69453_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/69453_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34625/69453_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEK93, HeLa, RH-30, RT4, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171004-HS6ST2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human HS6ST2
- Species Reactivity
- Human
- Applications
- ELISA, WB, FC, ADCC, Cyt, IHC, FuncS
- Related Disease
- Lung adenocarcinoma, small cell lung carcinoma, colorectal cancer, breast cancer, large cell lung carcinoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Versatile Use
The anti-HS6ST2 recombinant antibody TAB-0325CL has proven very versatile in our research applications such as ELISA, WB, FC, ADCC, Cyt, IHC, and FuncS. The high purity and strong binding to HS6ST2 ensure reliable results. Clear storage instructions and rigorous quality control make it a dependable reagent for cell surface glycoprotein studies.
Consistent Quality
I appreciate the consistency of the TAB-0325CL antibody when analyzing HS6ST2. Its performance in multiple applications such as Western Blot and ELISA supports studies on heparan sulfate proteoglycans. The detailed product description and high purity level allow for reliable and reproducible signaling pathway experiments.
Q&As
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Can the TAB-0325CL antibody be used in flow cytometry?
A: We have validated the TAB-0325CL antibody for use in flow cytometry among several other applications. Its high purity and specific reactivity to HS6ST2 ensure that it performs well across ELISA, WB, FC, ADCC, Cyt, IHC, and functional studies. The specifications and storage guidelines further enhance its reliability in flow cytometry.
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What is the verified purity level of the TAB-0325CL antibody?
A: We verify the purity of TAB-0325CL using SDS-PAGE alongside HPLC methods, consistently achieving results above 95%. This high purity is essential for its effective application in various assays such as ELISA, WB, and FC. The detailed documentation ensures proper use and optimal experimental outcomes in HS6ST2 related studies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HS6ST2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0323CL | Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0323CL) | ELISA, WB, FC, ADCC, Cyt, IHC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0326CL | Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0326CL) | ELISA, WB, FC, ADCC, Cyt, IHC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0327CL | Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0327CL) | ELISA, WB, FC, ADCC, Cyt, IHC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0328CL | Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0328CL) | ELISA, WB, FC, ADCC, Cyt, IHC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0329CL | Mouse Anti-HS6ST2 Recombinant Antibody (TAB-0329CL) | ELISA, WB, FC, ADCC, Cyt, IHC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC264 | Recombinant Anti-HS6ST2 Vesicular Antibody, EV Displayed (VS-0425-YC264) | ELISA, FC, Inhib, Cell-uptake |
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