Rabbit Anti-HSPA9 Recombinant Antibody (clone DS1698AB)
CAT#: MOR-1698
Recombinant Rabbit anti-HSPA9 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line A431
Immunofluorescent staining of human cell line A431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human HSPA9
- Species Reactivity
- Human
- Clone
- DS1698AB
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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