Human Anti-HTT Recombinant Antibody (clone C4) (CAT#: PABL-580)
Recombinant Human Antibody (C4) is capable of binding to HTT, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HTT mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HTT proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibits amyloid formation by exon1 fragments of huntingtin in vitro.
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Figure 1 Direct binding of C4 sFv to immobilized GST-fusion proteins analyzed by ELISA.
C4 sFv-myc-His6 antibodies were quantitated in microtiter plates coated with 70 nM of GST-fusion proteins and blocked with 1% BSA in PBS. Bound sFv were detected with anti-myc antibodies (9E10, 1 μg/ml), followed by alkaline phosphatase-conjugated goat anti-mouse IgG.
Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.
Figure 2 Immunofluorescent analysis of GFP-fusion HD fragments to the nucleus by anti-HD C4 sFv-HA-nls.
Immunofluorescence of HD-Q103-myc-His6 with C4 sFv-HA-nls (b, d, f) or a negative control sFv-HA-nls intrabody (a, c, e), after 48-h cotransfection in COS-7. HD-Q103-myc-His6 was detected by 9E10 mAb, followed by FITC-labeled goat anti-mouse IgG antibodies (c, d). The intrabodies were detected with polyclonal anti-HA antibodies, followed by rhodamine-labeled goat anti-rabbit IgG antibodies (a, b). (e, f) Dual staining of the same fields.
Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.
Specifications
- Immunogen
- Human huntingtin
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human HTT
- Species Reactivity
- Human
- Clone
- C4
- Applications
- Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1905-VHH | Recombinant Anti-human HTT VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| PABJ-0201 | Human Anti-HTT Recombinant Antibody (PABJ-0201) | ELISA, FuncS | Human VL |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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