Human Anti-HTT Recombinant Antibody (clone C4) (CAT#: PABL-580)

Figure 1 Direct binding of C4 sFv to immobilized GST-fusion proteins analyzed by ELISA.

C4 sFv-myc-His6 antibodies were quantitated in microtiter plates coated with 70 nM of GST-fusion proteins and blocked with 1% BSA in PBS. Bound sFv were detected with anti-myc antibodies (9E10, 1 μg/ml), followed by alkaline phosphatase-conjugated goat anti-mouse IgG.

Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.

Figure 2 Immunofluorescent analysis of GFP-fusion HD fragments to the nucleus by anti-HD C4 sFv-HA-nls.

Immunofluorescence of HD-Q103-myc-His6 with C4 sFv-HA-nls (b, d, f) or a negative control sFv-HA-nls intrabody (a, c, e), after 48-h cotransfection in COS-7. HD-Q103-myc-His6 was detected by 9E10 mAb, followed by FITC-labeled goat anti-mouse IgG antibodies (c, d). The intrabodies were detected with polyclonal anti-HA antibodies, followed by rhodamine-labeled goat anti-rabbit IgG antibodies (a, b). (e, f) Dual staining of the same fields.

Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.