Anti-Human Hepsin Recombinant Antibody scFv Fragment (Mab2.7.35) (CAT#: TAB-030CT-S(P))

Recombinant monoclonal antibody to Hepsin. This antibody intended for the prophylaxis and treatment of Prostate cancer.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 shows a further sensogram of an epitope mapping experiment.

Figure 1 shows a further sensogram of an epitope mapping experiment.

Here anti-hepsin antibody 2.7.35, 2.3.35 and 2.14.5 were immobilized on a CM5 chip and 50 nM hepsin was added. Then antibody 2.7.35 was injected with a concentration of 50 nM.

Figure 2 Comparison of inhibition of hepsin antibodies.

Figure 2 Comparison of inhibition of hepsin antibodies.

Inhibition of hepsin activity by antibody mouse Mab 2.7.35 (= mH35) (squares), chimeric Mab 2.7.35 (= chH35) (circles) and Mab55 (= hH35) (triangles). After preincubation of antibodies with hepsin for 30 minutes, hydrolysis reaction was started with addition of Ac-KQLR-AMC peptide. Fluorescence increase was measured after 40 minutes incubation. The IC50 values were calculated after fitting the % inhibition data to a four-parameter equation.

Figure 3 Binding of Mab55 (= hH35) to cell surface hepsin.

Figure 3 Binding of Mab55 (= hH35) to cell surface hepsin.

HEK293 cell lines that stably overexpress full length hepsin with a C-terminal GFP fusion tag were analyzed by flow cytometry. By incubation with increasing amounts of Mab55, specific and saturable surface staining could be detected.

Figure 4 Activity of hepsin antibodies against other serine proteases.

Figure 4 Activity of hepsin antibodies against other serine proteases.

Hepsin (1 nM), Matriptase (2 nM), HAT (2 nM), Enteropeptidase (0.7 nM), Trypsin (1.8 nM) were incubated with antibody (500 nM) for 30 minutes and reaction was started with 5 μΜ Ac-KQLR-AMC peptide.

Figure 5 Activity of antibody Mab55 analyzed in a FRET activity assay against human hepsin (blue), cynomolgus (brown) and mouse (magenta).

Figure 5 Activity of antibody Mab55 analyzed in a FRET activity assay against human hepsin (blue), cynomolgus (brown) and mouse (magenta).

Cross reactivity was found against cynomolgus, but not against mouse hepsin.

Figure 6 Progress curve of hepsin inhibition by Mab55 (= hH35).

Figure 6 Progress curve of hepsin inhibition by Mab55 (= hH35).

Hepsin (1 nM) was added to a mixture of Ac-KQLR-AMC peptide (10 μΜ) and 0, 111.1, 333.3 and 100 nΜ of Mab55. The increase of fluorescence was monitored at 10-second intervals over 200 seconds. The amidolytic activity of hepsin in absence of antibody shows a straight line (R² = 0.9946).


Specifications

  • Immunogen
  • EE-tagged human Hepsin
  • Host Species
  • Human
  • Derivation
  • Hybridoma cell line
  • Type
  • Chimeric antibody (mouse/human)
  • Specificity
  • Human
  • Species Reactivity
  • Human
  • Clone
  • Mab2.7.35
  • Applications
  • ELISA, FC, FuncS, Inhib, FRET
  • Related Disease
  • Prostate cancer

Applications

  • Application Notes
  • The antibody was validated for Inhibition, Flow cytometry, FRET and Function assay. For details, refer to Published Data.

Target

  • Alternative Names
  • TMPRSS1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone Mab2.7.35"

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Single-domain Antibody

CAT Product Name Application Type
NAB-1314-VHH Recombinant Anti-Human HPN VHH Single Domain Antibody ICC, FC, WB, FUNC Llama VHH

Recombinant Antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-030CT Anti-Human Hepsin Recombinant Antibody (Mab2.7.35) ELISA, Inhibition, FC Chimeric antibody (mouse/human)
TAB-030CT-F(E) Anti-Human Hepsin Recombinant Antibody Fab Fragment (Mab2.7.35) ELISA, Inhibition, FC Chimeric antibody (mouse/human)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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