Mouse Anti-Hev b 6.02 Recombinant Antibody (clone 6E7) (CAT#: PABZ-151)

Figure 1 ELISA inhibition assays

The average inhibition of IgE binding to solid-phase Hev b 6.02 was determined using isoform 2 and the chemically modified allergen with BPNS-skatole in sera from 10 pediatric patients. An average of 75% inhibition was found with isoform 2 and only 20% with the chemically modified allergen at the maximum concentration used. The inhibition curve with Hev b 6.02 as the inhibitor is shown for comparison.

Reyes-López, C. A., Hernández-Santoyo, A., Pedraza-Escalona, M., Mendoza, G., Hernández-Arana, A., & Rodrı́guez-Romero, A. (2004). Insights into a conformational epitope of Hev b 6.02 (hevein). Biochemical and biophysical research communications, 314(1), 123-130.

Figure 2 Specificity of the polyclonal and monoclonal anti-Hev b 6.02 antibodies in a dot blot assay.

The membranes were incubated with the rabbit and mouse IgGs. Proteins used are indicated in the left margin of both figures. BSA was used as negative control.

Reyes-López, C. A., Hernández-Santoyo, A., Pedraza-Escalona, M., Mendoza, G., Hernández-Arana, A., & Rodrı́guez-Romero, A. (2004). Insights into a conformational epitope of Hev b 6.02 (hevein). Biochemical and biophysical research communications, 314(1), 123-130.

Figure 3 Tryptophan fluorescence in the identification of Hev b 6.02 epitopes.

(A) Tryptophan intrinsic fluorescence of the complexes Hev b 6.02-recombinant antibodies at different molar ratios. (B) Stern–Volmer plots for acrylamide quenching effects on Hev b 6.02 and on its complexes with recombinant antibodies.

Pedraza-Escalona, M., Becerril-Luján, B., Agundis, C., Domínguez-Ramírez, L., Pereyra, A., Riaño-Umbarila, L., & Rodríguez-Romero, A. (2009). Analysis of B-cell epitopes from the allergen Hev b 6.02 revealed by using blocking antibodies. Molecular immunology, 46(4), 668-676.