Anti-Human Hla Recombinant Antibody (TAB-358CL) (CAT#: TAB-358CL)

1. Q 1: What are the primary applications of the Anti-Human Hla Recombinant Antibody in scientific research?

   A: The Anti-Human Hla Recombinant Antibody is primarily used in immunological research to study human leukocyte antigen (HLA) expression and function. This antibody is crucial for investigating immune responses, organ transplantation compatibility, and autoimmune disease mechanisms. Its high specificity and affinity make it a valuable tool in both basic and clinical research settings.

2. Q 2: How should the Anti-Human Hla Recombinant Antibody be stored to maintain its stability and activity?

   A: To ensure the stability and activity of the Anti-Human Hla Recombinant Antibody, it should be stored at -20°C or lower in aliquots to prevent repeated freeze-thaw cycles. Before use, thaw the aliquots on ice and gently mix. Proper storage conditions are critical to maintain the antibody's integrity and functionality over time.

3. Q 3: What protocols are recommended for using the Anti-Human Hla Recombinant Antibody in flow cytometry?

   A: For flow cytometry applications, it is recommended to use the Anti-Human Hla Recombinant Antibody at a concentration of 0.5-1 µg per million cells in 100 µL of staining buffer. Cells should be incubated with the antibody for 30 minutes at 4°C in the dark. After incubation, wash the cells twice with staining buffer before analyzing by flow cytometry.

4. Q 4: Can the Anti-Human Hla Recombinant Antibody be used in immunohistochemistry (IHC)? If so, what are the key considerations?

   A: Yes, the Anti-Human Hla Recombinant Antibody can be used in immunohistochemistry. For optimal results, antigen retrieval is recommended, typically using a citrate buffer (pH 6.0). The antibody should be diluted according to the specific IHC protocol, usually around 1:100 to 1:500. Incubation should be performed overnight at 4°C, followed by detection using an appropriate secondary antibody.

5. Q 5: What are the common issues faced when using the Anti-Human Hla Recombinant Antibody, and how can they be resolved?

   A: Common issues include non-specific binding, low signal, and high background. To resolve these, ensure optimal antibody dilution and proper blocking steps. Use fresh reagents and validate the antibody specificity with control samples. Additionally, optimizing the antigen retrieval process and incubation times can significantly improve results.

View the frequently asked questions answered by Creative Biolabs Support.