Anti-Human BAFF Recombinant Antibody (Tabalumab) (CAT#: TAB-216)
Recombinant monoclonal antibody to BAFF. Tabalumab (LY 2127399) is an anti-B-cell activating factor (BAFF) human monoclonal antibody designed for the treatment of autoimmune diseases and B cell malignancies.
Specific Inquiry
SDS-PAGE
Figure 1 Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in SDS-PAGE
Lane 1: Reduced mAb
Lane 2: Non-reduced mAb
SEC-HPLC
Figure 2 Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in SEC-HPLC
The purity of TAB-216-ADCP was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in WB
Western blot analysis of TAB-216-ADCP was performed by loading recombinant Human BAFF with his-tag.
TAB-216-ADCP incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-human IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
ELISA
Figure 4 Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in ELISA
ELISA analysis of TAB-216-ADCP was performed by coating with recombinant Human BAFF with his-tag.
The secondary antibody: HRP-Goat anti-human IgG
DB
Figure 5 Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in DB
Anti-Human BAFF Recombinant Antibody (Tabalumab), ADCP enhanced (TAB-216-ADCP) in DB
Dot Blot analysis of TAB-216-ADCP was performed by coating with recombinant Human BAFF with his-tag.
The secondary antibody: HRP-Goat anti-human IgG
SDS-PAGE
Figure 6 Anti-Human BAFF Recombinant Antibody (Tabalumab) in SDS-PAGE
SDS-PAGE analysis of TAB-216 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 7 Anti-Human BAFF Recombinant Antibody (Tabalumab) in SEC-HPLC
The purity of TAB-216 was greater than 95% as determined by SEC-HPLC.
WB
Figure 8 Anti-Human BAFF Recombinant Antibody (Tabalumab) in WB
Western blot analysis of TAB-216 was performed by loading human BAFF protein, His tag.
TAB-216 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L).
Lane 1: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 9 Anti-Human BAFF Recombinant Antibody (Tabalumab) in ELISA
ELISA analysis of TAB-216 was performed by coating with human BAFF protein (His tag). Then blocked with BSA and incubated with TAB-216. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG4 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- FuncS, IF, Neut, ELISA, FC, IP, IHC
- CAS
- 1143503-67-6
- Generic Name
- Tabalumab
- MW
- 146.25 kDa
- Related Disease
- Connective tissue disease
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Storage
- 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.
Applications
- Application Notes
- The TNFSF13B antibody has been reported in applications of Activ, Neut, PD.
Target
- Alternative Names
- Tabalumab;1143503-67-6;LY2127399;LY 2127399;TNFSF13B;tumor necrosis factor (ligand) superfamily, member 13b;TNFSF20;tumor necrosis factor ligand superfamily member 13B;BAFF;BLYS;CD257;TALL 1;TALL1;THANK;delta BAFF;Delta4 BAFF;B-lymphocyte stimulator;B-cel
- Gene ID
- 10673
- UniProt ID
- Q9Y275
Related Resources
Please refer to Tabalumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tabalumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Tabalumab"
Afuco™ Anti-TNFSF13B ADCC Recombinant Antibody (Tabalumab), ADCC EnhancedThis product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to BAFF. Tabalumab (LY 2127399) is an anti-B-cell activating factor (BAFF) human monoclonal antibody designed for the treatment of autoimmune diseases and B cell malignancies.
See other products for "TNFSF13B"
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| MOB-1226z | Mouse Anti-TNFSF13B Recombinant Antibody (clone 25D8) | WB, IP, IF, ELISA | Mouse IgM, κ |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-216, RRID: AB_3111877)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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