Anti-Human CD20 Recombinant Antibody (TAB-028)

Name: Anti-Human CD20 Recombinant Antibody (TAB-028)
SKU: TAB-028
Rating: 5 (1 reviews)

CAT#: TAB-028

Recombinant monoclonal antibody to Human CD20. This antibody is a human monoclonal antibody (for the CD20 protein) which appears to inhibit early-stage B lymphocyte activation. The immunogen of this antibody is Hmuan CD20 transfected NS/O cells.

Tested Data Gene Expression

Figure 1 Anti-CD20 Antibody (TAB-028) in Flow Cytometry

Flow cytometry analysis of Daudi cells with Anti-CD20 Antibody (Cat#TAB-028, Blue line) or isotype control antibody (Yellow line). A Goat Anti-human IgG (FITC) was used as the secondary antibody.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
Hmuan CD20 transfected NS/O cells.

Host Species
Human

Derivation
Human

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen

Species Reactivity
Human

Applications
Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, ICC and most other immunological methods.

MW
146.1 kDa

Related Disease
Rheumatoid arthritis (RA)

Product Property

Purity
>95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.

Target

Alternative Names
MS4A1; membrane-spanning 4-domains, subfamily A, member 1; CD20; B-lymphocyte antigen CD20; B1; Bp35; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell

Gene ID
931

UniProt ID
P11836

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(Creative Biolabs Cat# TAB-028, RRID: AB_3714256)

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Biosimilar Overview

Please refer to Ofatumumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ofatumumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-028.pdf

MSDS

MSDS - TAB-028.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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