What are the recommended applications for this antibody?

This recombinant monoclonal antibody is suitable for Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), and Flow Cytometry (FC). These applications enable detailed analyses of protein expression, function, and localization.

How does this antibody contribute to flow cytometry?

It allows precise detection and quantification of PD-L1 expression on human cells using flow cytometry. Its specificity affords low background noise and distinct separation between positive and negative populations.

Anti-Human CD274 Recombinant Antibody (TAB-418CQ)

CAT#: TAB-418CQ

Recombinant monoclonal antibody to CD274. This antibody is a humanized IgG1 monoclonal antibody that specifically binds to PD-L1, preventing it from interacting with its receptors (PD-1 and B7.1) on T-cells and antigen-presenting cells.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

❮

❯

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized

Type
IgG1, κ

Specificity
Human CD274

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry

Conjugate
Unconjugated

Related Disease
Cancers, non-small cell lung (NSCLC), Solid tumors, bladder, urothelial

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Target

Alternative Names
Programmed cell death 1 ligand 1; B7H1; B7-H1; PDL1; PD-L1; PDCD1L1; B7 homolog 1; B7 homologue 1

Gene ID
29126

UniProt ID
Q9NZQ7

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for TAB-418CQ. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Great Immunofluorescence Results

The antibody's effectiveness in IF allows detailed visualization of PD-L1 distribution at the cellular level. The exceptional clarity and low background ensure that our findings are sharp and unambiguous, greatly aiding in the interpretation of biological mechanisms.

Enhancing Cancer Biomarker Studies

Our studies focus on PD-L1 as a cancer biomarker. This antibody allows for comprehensive ELISA assays, enabling us to quantify expression levels with confidence and explore correlations with clinical outcomes, thereby driving impactful insights.

Q&As

What are the recommended applications for this antibody?

A: This recombinant monoclonal antibody is suitable for Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), and Flow Cytometry (FC). These applications enable detailed analyses of protein expression, function, and localization.
How does this antibody contribute to flow cytometry?

A: It allows precise detection and quantification of PD-L1 expression on human cells using flow cytometry. Its specificity affords low background noise and distinct separation between positive and negative populations.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-418CQ, RRID: AB_3111931)

Copy citation

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-418CQ.pdf

MSDS

MSDS - TAB-418CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Cd274"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
NAB-305-sdAb	Recombinant Anti-Mouse Cd274 VHH Single Domain Antibody	IHC, IP, FC, Neut, FUNC	Llama VHH

CAT	Product Name	Application	Type
MOB-1814z	Mouse Anti-CD274 Recombinant Antibody (clone 7A5)	FC, IHC, FuncS	Mouse IgG2b

CAT	Product Name	Application	Type
PABL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006)	WB, ELISA, FuncS	Human IgG

CAT	Product Name	Application	Type
PSBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment	WB, ELISA, FuncS	Human scFv

CAT	Product Name	Application	Type
PFBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment	WB, ELISA, FuncS	Human Fab

CAT	Product Name	Application	Type
TAB-0190CL	Anti-Human CD274 Recombinant Antibody (PD1-AB1)	FC, ELISA, FuncS

CAT	Product Name	Application	Type
TAB-0262CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Mouse IgG

CAT	Product Name	Application	Type
TAB-0263CL	Human Anti-CD274 Recombinant Antibody (TAB-0263CL)	ELISA, WB, SPR, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0264CL	Human Anti-CD274 Recombinant Antibody (TAB-0264CL)	ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib	Humanized IgG

CAT	Product Name	Application	Type
TAB-0265CL	Human Anti-CD274 Recombinant Antibody (TAB-0265CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0266CL	Human Anti-CD274 Recombinant Antibody (TAB-0266CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0267CL	Human Anti-CD274 Recombinant Antibody (TAB-0267CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0992CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL)	Inhib, FC, SPR	Mouse IgG

CAT	Product Name	Application	Type
TAB-0993CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL)	FC, SPR	Mouse IgG

CAT	Product Name	Application	Type
TAB-0994CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL)	ELISA, FC	Mouse IgG

CAT	Product Name	Application	Type
TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ

CAT	Product Name	Application	Type
TAB-0997CL	Anti-Human CD274 Recombinant Antibody (B60-55)	ELISA, FuncS, FC, PK	Human antibody

CAT	Product Name	Application	Type
TAB-1002CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL)	ELISA, FC	Mouse IgG, κ

CAT	Product Name	Application	Type
TAB-1003CL	Human Anti-CD274 Recombinant Antibody (TAB-1003CL)	FC, ELISA	Human IgG

CAT	Product Name	Application	Type
TAB-1004CL	Human Anti-CD274 Recombinant Antibody (TAB-1004CL)	FC	Human IgG

CAT	Product Name	Application	Type
TAB-1009CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL)	WB, IHC	Mouse IgG1, κ

CAT	Product Name	Application	Type
TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv

CAT	Product Name	Application	Type
TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
MOB-2354MZ	Mouse Anti-CD274 Recombinant Antibody (clone NJI3)	FC	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-324CQ	Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6)	IHC-P, FC, Neut, ICC	Mouse IgG2b, κ

CAT	Product Name	Application	Type
NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ

CAT	Product Name	Application	Type
NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ

CAT	Product Name	Application	Type
NEUT-327CQ	Mouse Anti-CD274 Recombinant Antibody (clone MIH1)	FC, FuncS, Neut, IHC, IF	Mouse IgG1, κ

CAT	Product Name	Application	Type
NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2

CAT	Product Name	Application	Type
NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ

CAT	Product Name	Application	Type
NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

CAT	Product Name	Application	Type
NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ

CAT	Product Name	Application	Type
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

CAT	Product Name	Application	Type
MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0193-YC	Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC)	ELISA	Humanized IgG4, κ

CAT	Product Name	Application	Type
HPAB-0194-YC	Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
HPAB-0193-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0194-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0195-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P))	SPR	Humanized scFv

CAT	Product Name	Application	Type
HPAB-0196-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P))	FC	Human scFv

CAT	Product Name	Application	Type
HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab

CAT	Product Name	Application	Type
HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab

CAT	Product Name	Application	Type
HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab

CAT	Product Name	Application	Type
HPAB-N0408-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH)	ELISA, FC, BL	Human VHH

CAT	Product Name	Application	Type
AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-N0307-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH)	ELISA, FC, BL	Humanized VHH

CAT	Product Name	Application	Type
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH

CAT	Product Name	Application	Type
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH

CAT	Product Name	Application	Type
VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer

CAT	Product Name	Application	Type
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

CAT	Product Name	Application	Type
VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

CAT	Product Name	Application	Type
VS-0825-YC8	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
VS-0825-YC47	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47)	ELISA, IHC, FC, IP, IF, Inhib	Human IgG1 kappa

CAT	Product Name	Application	Type
VS-1025-YC9	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9)	ISZ, Cyt, FuncS

CAT	Product Name	Application	Type
VS-1025-YC39	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC39)	ISZ, Cyt, FuncS

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Anti-ANGPT2 Recombinant Antibody (CAT# TAB-769)
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC

Anti-Human IL1Beta Recombinant Antibody (Canakinumab) (CAT# TAB-021)
Application: WB, ELISA, IP, FC, FuncS, Neut, IF

Human Anti-IL17RA Recombinant Antibody (TAB-134) (CAT# TAB-134)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Human Anti-ERBB3 Recombinant Antibody (TAB-189) (CAT# TAB-189)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Anti-Human Nogo-A Recombinant Antibody (TAB-903) (CAT# TAB-903)
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC

Anti-Human CD3E Recombinant Antibody (TAB-159) (CAT# TAB-159)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Anti-Human IL13 Recombinant Antibody (TAB-219) (CAT# TAB-219)
Application: ELISA, Neut, IF, IP, FC, FuncS

Anti-Human IL23 Recombinant Antibody (Tildrakizumab) (CAT# TAB-875)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Anti-Human MUC5AC Recombinant Antibody (TAB-200) (CAT# TAB-200)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Anti-Human TWEAK receptor Recombinant Antibody (TAB-178) (CAT# TAB-178)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Anti-Human Abeta Recombinant Antibody (TAB-229) (CAT# TAB-229)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC

Human Anti-SLC34A2 Recombinant Antibody (TAB-H45) (CAT# TAB-H45)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Anti-Human CD83 Recombinant Antibody (CAT# TAB-143CL)
Application:

Anti-Human DKK1 Recombinant Antibody (BHQ880) (CAT# TAB-214CL)
Application: IHC, ELISA, IB

Anti-Human GD2 Recombinant Antibody (hu14.18K322A) (CAT# TAB-304CL)
Application: FC, FuncS

Abciximab (CAT# TP-100CL)
Application:

Mouse Anti-UPAR Recombinant Antibody (clone ATN615) (CAT# PABC-130)
Application: ELISA, IP, WB, IHC, IF, FuncS

Mouse Anti-Il2 Recombinant Antibody (PABL-609) (CAT# PABL-609)
Application: FC, FuncS, IHC, ELISA

Mouse Anti-LAM Recombinant Antibody (clone CS-35) (CAT# PABW-158)
Application: ELISA, IF, FC

Mouse Anti-CD3 Recombinant Antibody (TAB-0416CL) (CAT# TAB-0416CL)
Application: FC, IB, ELISA

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.