Recombinant Mouse anti-Human CTTN Monoclonal antibody (T32)
CAT#: MOB-2286CT
Recombinant Mouse Monoclonall antibody to Human CTTN, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/1005_E8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11235/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to golgi apparatus, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/1421_D12_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/1015_E8_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/embranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57242/137089_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085733-CTTN
❮
❯
Specifications
- Immunogen
- Synthetic peptide from N terminal region of Human Cortactin
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- T32
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry paraffin embedded sections; Immunocytochemistry/Immunofluorescence; Flow Cytometry; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purity
- Tissue culture supernatant
- Format
- Liquid
- Buffer
- Preservative: 0.01% Sodium Azide. Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Target
- Alternative Names
- CTTN; cortactin; EMS1; src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate);
- Gene ID
- 2017
- UniProt ID
- Q14247
- Protein Refseq
- NP_001171669
- Function
- protein binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MRO-0380-CN | Rabbit Anti-CTTN Recombinant Antibody (clone CBACN-154) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0434F | Mouse Anti-CTTN Recombinant Antibody (ZG-0434F) | WB, IHC, ELISA | Mouse IgG |
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| VS3-WK954 | Rabbit Anti-CTTN Recombinant Antibody (VS3-WK954) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-HM790 | Mouse Anti-CTTN Recombinant Antibody (clone CBL188HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX305 | Mouse Anti-CTTN Recombinant Antibody (clone 401CT8.6.3) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC274 | CytoStream™ Rabbit Anti-CTTN Recombinant Antibody (VS13-YC274) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1792 | Anti-CTTN Immunohistochemistry Kit | IHC | |
| VS-0525-XY1793 | Anti-Mouse CTTN Immunohistochemistry Kit | IHC |
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