Anti-Human ERBB2 Recombinant Antibody (Trastuzumab Emtansine) (CAT#: TAB-H70)
Recombinant Humanized (from mouse) antibody to Human ERBB2. Trastuzumab emtansine (ado-trastuzumab emtansine, trade name trastuzumab emtansine) is an antibody-drug conjugate consisting of the monoclonal antibody trastuzumab (trastuzumab) linked to the cytotoxic agent mertansine (DM1). Trastuzumab alone stops growth of cancer cells by binding to the HER2/neu receptor, whereas mertansine enters cells and destroys them by binding to tubulin. Because the monoclonal antibody targets HER2, and HER2 is only over-expressed in cancer cells, the conjugate delivers the toxin specifically to tumor cells. The conjugate is abbreviated T-DM1.
Specific Inquiry
Figure 1 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.
Human epidermal growth factor receptor 2 (HER2) expression in N87 and N87-16-8 cells was analyzed by Western blotting.
Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.
Figure 2 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.
N87 and N87-16-8 cells were treated with 1 ug/mL DyLight 488 NHS Ester-labeled T-DM1 on ice for 1 h and washed three times with cold FACS buffer, after which binding of T-DM1 to cells was analyzed by flow cytometry.
Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.
Specifications
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Specificity
- ERBB2(epidermal growth factor receptor 2, receptor tyrosine-protein kinase erbB-2, EGFR2, HER2, HER-2, p185c-erbB2, NEU, CD340) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- ELISA, IP, FC, FuncS, Neut, IF, ICC
- Trade name
- trastuzumab emtansine
- CAS
- 1018448-65-1
- Generic Name
- trastuzumab emtansine
- Biological Half-Life
- 4 days
- ATC Code
- L01XC14
- UNII
- SE2KH7T06F
- MW
- 148.5 kDa
- Related Disease
- Breast cancers overexpressing ERBB2, metastatic
Product Property
- Purity
- >95.0% as determined by analysis by RP-HPLC.
Applications
- Application Notes
- The ERBB2 antibody has been reported in applications of WB, FC.
Target
- Alternative Names
- trastuzumab emtansine;trastuzumab emtansine;1018448-65-1;PRO132365;trastuzumab-MCC-DM1;Trastuzumab-DM1;T-DM1;RG3502;trastuzumab emtansine;ado-trastuzumab emtansine;ERBB2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian);NG
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
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(Creative Biolabs Cat# TAB-H70, RRID: AB_3112043)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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