Anti-Human ERBB2 Therapeutic Antibody (Trastuzumab Emtansine) (CAT#: TAB-H70)

Recombinant Humanized (from mouse) antibody to Human ERBB2. Trastuzumab emtansine (ado-trastuzumab emtansine, trade name trastuzumab emtansine) is an antibody-drug conjugate consisting of the monoclonal antibody trastuzumab (trastuzumab) linked to the cytotoxic agent mertansine (DM1). Trastuzumab alone stops growth of cancer cells by binding to the HER2/neu receptor, whereas mertansine enters cells and destroys them by binding to tubulin. Because the monoclonal antibody targets HER2, and HER2 is only over-expressed in cancer cells, the conjugate delivers the toxin specifically to tumor cells. The conjugate is abbreviated T-DM1.

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Figure 1 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

Human epidermal growth factor receptor 2 (HER2) expression in N87 and N87-16-8 cells was analyzed by Western blotting.

Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.

Figure 2 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

N87 and N87-16-8 cells were treated with 1 ug/mL DyLight 488 NHS Ester-labeled T-DM1 on ice for 1 h and washed three times with cold FACS buffer, after which binding of T-DM1 to cells was analyzed by flow cytometry.

Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Specificity
  • ERBB2(epidermal growth factor receptor 2, receptor tyrosine-protein kinase erbB-2, EGFR2, HER2, HER-2, p185c-erbB2, NEU, CD340) [Homo sapiens]
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IP, FC, FuncS, Neut, IF, ICC
  • Trade name
  • trastuzumab emtansine
  • CAS
  • 1018448-65-1
  • Generic Name
  • trastuzumab emtansine
  • Biological Half-Life
  • 4 days
  • ATC Code
  • L01XC14
  • UNII
  • SE2KH7T06F
  • MW
  • 148.5 kDa
  • Related Disease
  • Breast cancers overexpressing ERBB2, metastatic

Applications

  • Application Notes
  • The ERBB2 antibody has been reported in applications of WB, FC.

Target

  • Alternative Names
  • trastuzumab emtansine;trastuzumab emtansine;1018448-65-1;PRO132365;trastuzumab-MCC-DM1;Trastuzumab-DM1;T-DM1;RG3502;trastuzumab emtansine;ado-trastuzumab emtansine;ERBB2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian);NG

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "ERBB2"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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