Recombinant Mouse anti-Human HTT Monoclonal antibody (3502D2b)

Name: Recombinant Mouse anti-Human HTT Monoclonal antibody (3502D2b)
SKU: MOB-0905CT
Rating: 5 (1 reviews)

CAT#: MOB-0905CT

Recombinant Mouse monoclonal antibody to Human HTT, expressed in Chinese Hamster Ovary cells(CHO).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg

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Specifications

Immunogen
Recombinant fragment (Human) from an internal region of Huntingtin.

Host Species
Mouse

Species Reactivity
Human

Clone
3502D2b

Applications
Used for immunoassay techniques such as: Dot blot; Western Blot

Conjugate
Unconjugated

Product Property

Purity
Protein G purified

Format
Liquid

Concentration
0.1 mg/ml

Buffer
Preservative: 0.05% Sodium Azide Constituents: 1% BSA, PBS (8mM Dibasic monohydrogen sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Monobasic dihydrogen sodium phosphate), pH 7.4

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

Alternative Names
HTT; huntingtin; HD; IT15; huntington disease protein;

Gene ID
3064

UniProt ID
P42858

Protein Refseq
NP_002102

Function
beta-tubulin binding; dynactin binding; dynein intermediate chain binding; p53 binding; protein binding; transcription factor binding;

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-0905CT.pdf

MSDS

MSDS - MOB-0905CT.pdf

COA

Certificate of Analysis Lookup

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