Anti-Human IGHE Recombinant Antibody (Omalizumab)
CAT#: TAB-007
Recombinant monoclonal antibody to Human IgE. Omalizumab is a recombinant DNA-derived humanized IgG1k monoclonal antibody that specifically binds to free human immunoglobulin E (IgE) in the blood and interstitial fluid and to membrane-bound form of IgE (mIgE) on the surface of mIgE-expressing B lymphocytes. Unlike an ordinary anti-IgE antibody, omalizumab does not bind to IgE that is already bound by the high affinity IgE receptor (FcεRI) on the surface of mast cells, basophils, and antigen-presenting dendritic cells. IgE is commonly involved in type I hypersensitivity, which manifests the most prevalent allergic diseases. It has been estimated that as high as 20 to 40% of the populations who live a western lifestyle in economically advanced countries are affected by allergy and seek medical help. In the U.S., 8% of adults and 10% of children have asthma. While allergy occurs more frequently in individuals with higher serum IgE levels, such a correlation is only statistical and not absolute. Some allergic individuals have very low serum IgE, and some people with very high IgE have no allergic problems.
Published Data
Gene Expression
Figure 2 A single IgE mutation prevents omalizumab binding.
SPR-binding assays with immobilized omalizumab.
Pennington, L. F., Tarchevskaya, S., Brigger, D., Sathiyamoorthy, K., Graham, M. T., Nadeau, K. C., ... & Jardetzky, T. S. (2016). Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange. Nature communications, 7, 11610.
Figure 3 E2_79 and omalizumab bind to both WT and Cys-335 IgE-Fc proteins.
A , E2_79 binds to both WT IgE-Fc ( open circles , solid line ) and Cys-335 IgE-Fc ( filled circles , dashed line ) in an ELISA assay. IgE-Fc proteins were plated and incubated with E2_79 at the indicated concentrations, and E2_79 binding was detected with an anti-His tag antibody. B , omalizumab binds to both WT IgE-Fc ( open circles , solid line ) and Cys-335 IgE-Fc ( filled circles , dashed line ) in an ELISA assay. IgE-Fc proteins were plated and incubated with omalizumab, and omalizumab binding was detected with a polyclonal anti-IgG antibody. C and D , side ( C ) and top ( D ) views of the omalizumab-binding region on the IgE-Fc C ⑀ 3 domain. The main chain conformation of the IgE-Fc Cys-335 structure and the disulfide bond at residue 335 are shown in magenta , whereas residues mapped to the omalizumab binding region are shown in stick representation . Omali- zumab binding interactions have been mapped to IgE residues 421– 432 including the FG loop residues 424 – 426 (HLP) that lie within the Fc ⑀ RI ␣ binding interface (33). Despite the conformational restriction imposed by the Cys-335 disulfide, the omalizumab epitope remains accessible to binding.
Wurzburg, B. A., Kim, B., Tarchevskaya, S. S., Eggel, A., Vogel, M., & Jardetzky, T. S. (2012). An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation. Journal of biological chemistry, jbc-M112.
Figure 4 Omalizumab has no inhibitory effect on anti-IgE-induced degranulation.
(a) Freshly isolated human skin mast cells were coincubated with or without omalizumab (10 ug/mL) for 24 h or with DARPin Fc (100 pmol/mL) for 1 h as positive control for FceRI-FccRIIb cross linking. Mast cells were then activated with anti-IgE (1 ug/mL) for 1 h and histamine release determined. Results are expressed as mean SEM histamine release (% total) of three independent experiments. Panel (b) shows peripheral blood-derived monocytes from healthy donors that were coincubated with or without omalizumab (10 ug/mL) for 1 h and then activated using anti-IgE (5 ug/mL) for another hour to induce degranulation. Results are expressed as mean SEM histamine release (% total) of five independent experiments.
Gericke, J., Ohanyan, T., Church, M. K., Maurer, M., & Metz, M. (2015). Omalizumab may not inhibit mast cell and basophil activation in vitro. Journal of the European Academy of Dermatology and Venereology, 29(9), 1832-1836.
❮
❯
RNA Expression
Figure 1 RNA cell line category: Cell line enriched (U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211891-IGHE
❮
❯
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen
- Species Reactivity
- Human
- Applications
- WB, ELISA, IP, FC, FuncS, Neut, IF
- Trade name
- omalizumab
- CAS
- 242138-07-4
- Generic Name
- Omalizumab
- Biological Half-Life
- 26 days
- ATC Code
- R03DX05
- DrugBank
- DB00043
- UNII
- 2P471X1Z11
- ChEMBL
- CHEMBL1201589
- MW
- 145,058.2 g/mol
- Related Disease
- Chronic idiopathic urticaria
Product Property
- Purity
- >95%, by SDS-PAGE with silver staining, under reducing conditions.
- Storage
- At -20°C for one year.
Applications
- Application Notes
- The IGHE antibody has been reported in applications of FuncS, SPR, ELISA, Inhib, FC.
Target
- Alternative Names
- Omalizumab;omalizumab;242138-07-4;DB00043IGHE;Immunoglobulin heavy constant epsilon;
- Gene ID
- 28223
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for TAB-007. Click the button above to contact us or submit your feedback about this product.
Great for IgE Detection
I have incorporated the recombinant anti-Human IGHE antibody into my studies focusing on IgE detection. The antibody, being humanized and highly purified (>95% by SDS-PAGE), has provided reliable results in ELISA and western blots, making it an asset in my research protocols.
Reliable and Robust
The performance of this humanized IgE antibody is impressive in various immunoassays including IP and FC. Its consistent purity and reliable binding to free human IgE have enhanced the accuracy of our experiments. I am pleased with the reproducibility and technical support provided.
Q&As
-
Will this antibody work in ELISA and FC assays?
A: Yes, the recombinant anti-Human IGHE antibody is validated for ELISA, flow cytometry, and other immunoassays. Its humanized design and high purity ensure that it reliably binds free human IgE, producing consistent and reproducible signals in diverse assay systems.
-
What are the key attributes that support its use in research?
A: The antibody's high specificity for free human IgE, combined with its purification resulting in>95% purity, makes it highly reliable. Its validation across ELISA, WB, IP, and FC applications ensures that it meets critical research requirements for protein detection.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Biosimilar Overview
Please refer to Omalizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Omalizumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Omalizumab"
Afuco™ Anti-IGHE ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-007)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human IgE. It is a recombinant DNA-derived humanized IgG1k monoclonal antibody that specifically binds to free human immunoglobulin E (IgE) in the blood and interstitial fluid and to membrane-bound form of IgE (mIgE) on the surface of mIgE-expressing B lymphocytes.
DrugMonitor™ Anti-Omalizumab Antibody (VS-1224-YC841)Omalizumab is a monoclonal antibody targeting immunoglobulin E (IgE), and it is prescribed for asthma, chronic idiopathic urticaria, chronic rhinosinusitis with nasal polyps, and IgE-mediated food allergies. The DrugMonitor™ Anti-Omalizumab Antibody (VS-1224-YC841) is an anti-drug antibody (ADA) against Omalizumab. This drug-based antibody is raised in mice immunized with the Omalizumab. The anti-Omalizumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Omalizumab in samples.
See other products for "IGHE"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1075z | Mouse Anti-IGHE Recombinant Antibody (clone 20G9) | WB, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-092 | Recombinant Anti-human IGHE VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-755 | Anti-IGHE Recombinant Antibody (TAB-755) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L061R | anti-IGHE immunotoxin (Fab)-RTA | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-256CQ | Human Anti-IGHE Recombinant Antibody (TAB-256CQ) | Agonist | Human IgG2, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-257CQ | Human Anti-IGHE Recombinant Antibody (TAB-257CQ) | Agonist | Human IgG2, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-258CQ | Human Anti-IGHE Recombinant Antibody (TAB-258CQ) | Agonist | Human IgG2, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-256CQ-S(P) | Human Anti-IGHE Recombinant Antibody; scFv Fragment (TAB-256CQ-S(P)) | ELISA, FC, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-257CQ-S(P) | Human Anti-IGHE Recombinant Antibody; scFv Fragment (TAB-257CQ-S(P)) | ELISA, FC, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0168-LSX | Mouse Anti-IGHE Recombinant Antibody (clone MaE10) | ELISA, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0169-LSX | Mouse Anti-IGHE Recombinant Antibody (clone MAE11) | ELISA, FC, SPR | Mouse IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-LSX | Human Anti-IGHE Recombinant Antibody (clone 235-5.2) | ELISA, FC | Humanized IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0171-LSX | Human Anti-IGHE Recombinant Antibody (HPAB-0171-LSX) | ELISA, RIA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0168-LSX-S(P) | Mouse Anti-IGHE Recombinant Antibody (clone MaE10); scFv Fragment | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0169-LSX-S(P) | Mouse Anti-IGHE Recombinant Antibody (clone MAE11); scFv Fragment | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-LSX-S(P) | Human Anti-IGHE Recombinant Antibody (clone 235-5.2); scFv Fragment | ELISA, FC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0171-LSX-S(P) | Human Anti-IGHE Recombinant Antibody; scFv Fragment (HPAB-0171-LSX-S(P)) | ELISA, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0172-LSX-S(P) | Human Anti-IGHE Recombinant Antibody; scFv Fragment (HPAB-0172-LSX-S(P)) | ELISA, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0168-LSX-F(E) | Mouse Anti-IGHE Recombinant Antibody (clone MaE10); Fab Fragment | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0169-LSX-F(E) | Mouse Anti-IGHE Recombinant Antibody (clone MAE11); Fab Fragment | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-LSX-F(E) | Human Anti-IGHE Recombinant Antibody (clone 235-5.2); Fab Fragment | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0171-LSX-F(E) | Human Anti-IGHE Recombinant Antibody; Fab Fragment (HPAB-0171-LSX-F(E)) | ELISA, RIA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0172-LSX-F(E) | Human Anti-IGHE Recombinant Antibody; Fab Fragment (HPAB-0172-LSX-F(E)) | ELISA, RIA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-007 | Afuco™ Anti-IGHE ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-007) | ELISA, IP, FC, FuncS, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-255 | Afuco™ Anti-IGHE ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-255) | IP, IF, FuncS, FC, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-755 | Afuco™ Anti-IGHE ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-755) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY120 | Human Anti-IGHE (clone Omalizumab) scFv-Fc Chimera | ELISA, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC368 | Recombinant Anti-IGHE Vesicular Antibody, EV Displayed (VS-0425-YC368) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3456 | Anti-Sheep IGHE Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3457 | Anti-Horse IGHE Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC218 | Recombinant Anti-IGHE (HLP motif x FcεRI binding site) Biparatopic Antibody, Tandem scFv | ELISA, FC, IF, IP, Neut | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC169 | SmartAb™ Recombinant Anti-IGHE pH-dependent Antibody (Clone MaE10) | ELISA, FC | Mouse IgG1 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, Cyt, Stim, PP, Agonist
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: Neut, ELISA, FuncS
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
