Human Anti-IL17RA Recombinant Antibody (TAB-134) (CAT#: TAB-134)
This product is a recombinant Human antibody that recognizes IL17RA. The antibody was purified by affinity chromatography.
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Custom Production
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ELISA
Figure 1 Anti-Human IL17RA Antibody (TAB-134) in ELISA
ELISA analysis of TAB-134 was performed by coating with Human IL17RA Protein (His Tag). Then blocked with BSA and incubated with TAB-134. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human IL17RA Antibody (TAB-134) in WB
Western blot analysis of TAB-134 was performed with Human IL17RA Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-134 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human IL17RA Antibody (TAB-134) in DB
Dot Blot analysis of TAB-134 was performed by coating with Human IL17RA Protein (His Tag).
TAB-134 incubation concentration: 2 μg/mL.
HRP goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG2, κ
- Species Reactivity
- Human
- Applications
- Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, ICC and most other immunological methods.
- MW
- 144.06 kDa
- Related Disease
- Psoriatic arthritis (PSA)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-093 | Recombinant Anti-human IL17RA VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-134, RRID: AB_3111829)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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