Anti-Human IL5RA Recombinant Antibody (Benralizumab) (CAT#: TAB-222)

Recombinant monoclonal antibody to IL5RA. Benralizumab (INN) is a monoclonal antibody for the treatment of asthma. It is directed against the alpha-chain of the interleukin-5 receptor (CD125).


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SDS-PAGE

Figure 1 Anti-Human IL5RA Antibody (TAB-222) in SDS-PAGE

Figure 1 Anti-Human IL5RA Antibody (TAB-222) in SDS-PAGE

SDS-PAGE analysis of TAB-222 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

HPLC

Figure 2 Anti-Human IL5RA Antibody (TAB-222-LowE) in HPLC

Figure 2 Anti-Human IL5RA Antibody (TAB-222-LowE) in HPLC

The purity of this antibody is> 95% as determined by SEC-HPLC.

WB

Figure 3 Anti-Human IL5RA Antibody (TAB-222) in WB

Figure 3 Anti-Human IL5RA Antibody (TAB-222) in WB

Western blot analysis of TAB-222 was performed by loading IL5RA Protein, Human, Recombinant (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-222 and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 4 Anti-Human IL5RA Antibody (TAB-222) in ELISA

Figure 4 Anti-Human IL5RA Antibody (TAB-222) in ELISA

ELISA analysis of TAB-222 was performed by coating with IL5RA Protein, Human, Recombinant (His Tag). Then blocked with BSA, and incubated with Anti-Human IL5RA Antibody (TAB-222). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

DB

Figure 5 Anti-Human IL5RA Antibody (TAB-222) in DB

Figure 5 Anti-Human IL5RA Antibody (TAB-222) in DB

Dot Blot analysis of TAB-222 was performed by coating with IL5RA Protein, Human, Recombinant (His Tag).

TAB-222 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • Suitable for use in WB, FC, IP, ELISA, Neut, FuncS, IF and most other immunological methods.
  • CAS
  • 1044511-01-4
  • Generic Name
  • Benralizumab
  • UNII
  • 71492GE1FX
  • MW
  • 146.0 kDa
  • Related Disease
  • Asthma

Product Property

  • Purity
  • >95.0% as determined by analysis by RP-HPLC.
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • Benralizumab;1044511-01-4;MEDI-563;BIW-8405;KHK4563;IL5RA;interleukin 5 receptor, alpha;IL5R;interleukin-5 receptor subunit alpha;CD125;CDw125;CD125 antigen;IL-5R subunit alpha;IL-5 receptor subunit alpha;interleukin 5 receptor type 3;interleukin-5 recept

Related Resources

  • Biosimilar Overview
Please refer to Benralizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Benralizumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Benralizumab"

Afuco™ Anti-IL5RA ADCC Recombinant Antibody (Benralizumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to IL5RA. Benralizumab (INN) is a monoclonal antibody for the treatment of asthma. It is directed against the alpha-chain of the interleukin-5 receptor (CD125).

See other products for "IL5RA"

Recombinant Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1813 Rabbit Anti-IL5RA Recombinant Antibody (clone DS1813AB) WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-222 Afuco™ Anti-IL5RA ADCC Recombinant Antibody (Benralizumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS ADCC enhanced antibody

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-1545WJ-F(E) Human Anti-IL5RA Recombinant Antibody; Fab Fragment (clone KM1399) ELISA, WB Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1545WJ-S(P) Human Anti-IL5RA Recombinant Antibody; scFv Fragment (clone KM1399) ELISA, WB Human scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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