Goat Anti-Human IgG Fab Antibody (HRP)

Customer Review

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Emily Smith
05.Jan,24

Great for Detection

The Anti-Human IgG Fab Antibody (HRP) has been excellent for our detection assays. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

David Jones
27.May,23

Effective and Dependable

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for human IgG Fab is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Jessica Davis
30.Sep,22

Consistent High-Quality Results

This antibody has proven to be consistent and dependable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for human IgG Fab have been particularly beneficial.

Q&As

1. Is the anti-Human IgG Fab antibody HRP conjugated suitable for use in Western blotting?

   A: Yes, the anti-Human IgG Fab antibody HRP conjugated (MOB-0361MC) is suitable for use in Western blotting. It provides specific binding and HRP conjugation allows for easy detection in Western blot assays.

2. What is the recommended storage condition for the anti-Human IgG Fab antibody HRP conjugated?

   A: The recommended storage condition for the anti-Human IgG Fab antibody HRP conjugated (MOB-0361MC) is at 2-8°C. It should be kept in a tightly sealed vial to prevent contamination and degradation.

3. Can the anti-Human IgG Fab antibody HRP conjugated be used in ELISA applications?

   A: Yes, the anti-Human IgG Fab antibody HRP conjugated (MOB-0361MC) can be used in ELISA applications. It has been validated for use in such assays and provides reliable detection of Human IgG Fab fragments.

4. Is the anti-Human IgG Fab antibody HRP conjugated effective in immunofluorescence assays?

   A: Yes, the anti-Human IgG Fab antibody HRP conjugated (MOB-0361MC) is effective in immunofluorescence assays. It can be used to visualize IgG Fab fragments in cells and tissues using HRP-based detection methods.

5. What is the optimal dilution for using the anti-Human IgG Fab antibody HRP conjugated in Western blotting?

   A: The optimal dilution for using the anti-Human IgG Fab antibody HRP conjugated (MOB-0361MC) in Western blotting is typically 1:1000 to 1:5000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Gehrlein, Alexandra, et al. "Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct." Nature communications 14.1 (2023): 2057. https://doi.org/s41467-023-37632-4
   This study investigates the development of a therapeutic strategy targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase (GCase) deficiency, which is linked to Gaucher's disease and Parkinson's disease. Researchers engineered a brain-penetrant enzyme construct, GCase-BS, combining β-glucocerebrosidase with a transferrin receptor-binding moiety to facilitate its passage across the blood-brain barrier. The GCase-BS construct showed improved lysosomal uptake and function, effectively reducing pathological lipid accumulations in cellular and mouse models.
   Creative Biolabs provided the HRP-conjugated pAb anti-hFab(CH1) antibody (Cat# MOB-0361MC) used in this study. This antibody was essential for measuring IgG levels of the GCase-BS molecules after incubation in mouse plasma. By enabling accurate detection and quantification of IgG levels, the antibody from Creative Biolabs supported the researchers in assessing both the enzymatic activity of GCase and the stability of the GCase-BS constructs, thereby contributing to the evaluation of their therapeutic potential.
2. Roubidoux, Ericka Kirkpatrick, et al. "Utility of nasal swabs for assessing mucosal immune responses towards SARS-CoV-2." Scientific Reports 13.1 (2023): 17820. https://doi.org/s41598-023-44989-5
   This study investigates the utility of nasal swabs for assessing mucosal immune responses to SARS-CoV-2. The researchers developed a novel methodology to measure cytokine and antibody responses in the nasal epithelium using nasopharyngeal swabs collected before and after SARS-CoV-2 infection or vaccination. They compared these mucosal responses to systemic responses, finding distinct cytokine profiles in nasal versus plasma samples. The study also examined the kinetics of nasal IgA and IgG antibodies post-infection and vaccination, highlighting the importance of mucosal immunity in respiratory infections.
   Creative Biolabs provided the HRP-conjugated anti-human IgG antibody (Cat# MOB-0361MC) used in the total IgG ELISA assays. This antibody was crucial for detecting and quantifying IgG levels in nasal swab samples, allowing the researchers to accurately measure SARS-CoV-2 specific IgG responses. By enabling precise quantification of IgG, the antibody from Creative Biolabs supported the study's exploration of the dynamics of mucosal antibody responses, contributing to a better understanding of immunity at the nasal epithelium.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-0361MC, RRID: AB_3111475)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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