Recombinant Mouse anti-Human MBD1 Monoclonal antibody (2C7)
CAT#: MOB-1223CT
Recombinant Mouse monoclonal antibody to Human MBD1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68850/1715_A5_8_cr57fd2701ec147_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/923_F1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_2_1_selected.jpg
Normal Tissue
Figure 4 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36003/71834_A_5_5_selected.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9017/33413_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141644-MBD1
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Specifications
- Immunogen
- Recombinant fragment: HHLGPTLKPT LATRTAQPDH TQAPTKQEAG GGFVLPPPGT DLVFLREGAS SPVQVPGPVA ASTEALLQEA QCSGLSWVVA LPQVKQEKAD TQDE, corresponding to amino acids 415-508 of Human MBD1, with a 26 kDa proprietary tag.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 2C7
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein A purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None Constituents: 1X PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- MBD1; methyl-CpG binding domain protein 1; RFT; PCM1; CXXC3; methyl-CpG-binding domain protein 1; CXXC-type zinc finger protein 3; protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription;
- Gene ID
- 4152
- UniProt ID
- Q9UIS9
- Protein Refseq
- NP_001191065
- Function
- DNA binding; metal ion binding; methyl-CpG binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; zinc ion binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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