Recombinant Mouse anti-Human MID1 Monoclonal antibody (EML512)
CAT#: MOB-1236CT
Recombinant Mouse monoclonal antibody to Human MID1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line GAMG
Immunofluorescent staining of human cell line GAMG shows localization to cytosol, the Golgi apparatus & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2878/2032_G2_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3715/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3715/10877_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3715/10877_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3715/10877_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3715/10877_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, AF22)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101871-MID1
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Specifications
- Immunogen
- Recombinant fragment: PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR , corresponding to amino acids 441-540 of Human MID1
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML512
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- MID1; midline 1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18; E3 ubiquitin-protein ligase Midline-1; Opitz/BBB syndrome; RING finger protein 59; RING finger protein Midline-1; midline 1 RING finger protein; tripartite motif protein TRIM18; putative transcription factor XPRF; tripartite motif-containing protein 18; zinc finger on X and Y, mouse, homolog of;
- Gene ID
- 4281
- UniProt ID
- O15344
- Protein Refseq
- NP_000372
- Function
- ligase activity; metal ion binding; ubiquitin protein ligase binding; zinc ion binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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