Recombinant Mouse anti-Human NEDD9 Monoclonal antibody (25B22) (CAT#: MOB-0154CT)
Recombinant Mouse monoclonal antibody to Human NEDD9, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9689/1979_C10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9689/1979_C10_1_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39270/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39270/if_selected.jpg
Figure 3 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72955/170255_B_1_4_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72955/170255_B_1_4_selected.jpg
Figure 4 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells and decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9720/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells and decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9720/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_8_8.jpg
Figure 6 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_3.jpg
Figure 7 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, HSkMC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111859-NEDD9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111859-NEDD9
Specifications
- Immunogen
- Fusion protein, corresponding to amino acids 82-398 of Human HEF1.
- Host Species
- Mouse
- Specificity
- Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. Does not recognize p130 Cas. Has not been tested on Sin1.
- Species Reactivity
- Human
- Clone
- 25B22
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: 0.1% Sodium Azide Constituents: PBS, pH 7.2
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- NEDD9; neural precursor cell expressed, developmentally down-regulated 9; CAS2; CASL; HEF1; CAS-L; CASS2; enhancer of filamentation 1; cas-like docking; p130Cas-related protein; Enhancer of filamentation 1 p55; renal carcinoma antigen NY-REN-12; Cas scaffolding protein family member 2; Crk-associated substrate related protein Cas-L; neural precursor cell expressed developmentally down-regulated protein 9;
- Gene ID
- 4739
- UniProt ID
- Q14511
- Protein Refseq
- NP_001135865
- Function
- protein binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2741z | Mouse Anti-NEDD9 Recombinant Antibody (clone 12C6) | WB, ELISA, ICC, IF, IP | Mouse IgG2b, κ |
| VS1-YJ87 | Mouse Anti-NEDD9 Recombinant Antibody (clone 2G9) | IF, IHC-P, WB, IP | Mouse IgG1, κ |
| VS1-YJ88 | Mouse Anti-NEDD9 Recombinant Antibody (clone 14A11) | IF, IP | Mouse IgG2b, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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