Recombinant Mouse anti-Human NEDD9 Monoclonal antibody (25B22) (CAT#: MOB-0154CT)
Recombinant Mouse monoclonal antibody to Human NEDD9, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9689/1979_C10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9689/1979_C10_1_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39270/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39270/if_selected.jpg
Figure 3 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72955/170255_B_1_4_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72955/170255_B_1_4_selected.jpg
Figure 4 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells and decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9720/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells and decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9720/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_8_8.jpg
Figure 6 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_3.jpg
Figure 7 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38768/119176_A_7_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, HSkMC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111859-NEDD9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111859-NEDD9
Specifications
- Immunogen
- Fusion protein, corresponding to amino acids 82-398 of Human HEF1.
- Host Species
- Mouse
- Specificity
- Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. Does not recognize p130 Cas. Has not been tested on Sin1.
- Species Reactivity
- Human
- Clone
- 25B22
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: 0.1% Sodium Azide Constituents: PBS, pH 7.2
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- NEDD9; neural precursor cell expressed, developmentally down-regulated 9; CAS2; CASL; HEF1; CAS-L; CASS2; enhancer of filamentation 1; cas-like docking; p130Cas-related protein; Enhancer of filamentation 1 p55; renal carcinoma antigen NY-REN-12; Cas scaffolding protein family member 2; Crk-associated substrate related protein Cas-L; neural precursor cell expressed developmentally down-regulated protein 9;
- Gene ID
- 4739
- UniProt ID
- Q14511
- Protein Refseq
- NP_001135865
- Function
- protein binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2741z | Mouse Anti-NEDD9 Recombinant Antibody (clone 12C6) | WB, ELISA, ICC, IF, IP | Mouse IgG2b, κ |
| VS1-YJ87 | Mouse Anti-NEDD9 Recombinant Antibody (clone 2G9) | IF, IHC-P, WB, IP | Mouse IgG1, κ |
| VS1-YJ88 | Mouse Anti-NEDD9 Recombinant Antibody (clone 14A11) | IF, IP | Mouse IgG2b, κ |
| VS7-0425-WR461 | Mouse Anti-NEDD9 Recombinant Antibody (clone 3F7A9) | WB, ICC, FC | Mouse IgG1 |
| VS-0625-FY1441 | Human Anti-NEDD9 Recombinant Antibody (clone 2506W1336) | ELISA, Spiked IP, IF | Human IgG1 |
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