Anti-Human NT5E Recombinant Antibody (TAB-441CQ)
CAT#: TAB-441CQ
Recombinant monoclonal antibody to NT5E. This product is a human monoclonal antibody that targets NT5E.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
Normal Tissue
Figure 7 Cervix
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1, λ
- Specificity
- Human 5'-nucleotidase
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Solid Tumours, Pancreatic and Colorectal cancer
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
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Consistent Performance Across Assays
We've used the Anti-Human NT5E Antibody across several platforms. It consistently performs well, providing reliable results. Its recombinant nature reduces variability, making it indispensable in our ongoing cancer research.
Superior for Tumor Studies
This antibody has been critical in our tumor profiling endeavors. By delivering precise targeting for NT5E, it has allowed us to derive insightful relationships between enzyme activity and tumor spread.
Enhancing Understanding of NT5E Interactions
Through co-immunoprecipitation techniques aided by this antibody, we've uncovered novel NT5E interaction partners, driving forward our understanding of its role in cellular signaling.
Flexible Use Across Protocols
Its flexible application across different assay types allows seamless integration into multiple research projects, from basic science to applied clinical research contexts.
Supports Robust Flow Cytometry Analysis
In flow cytometry, this antibody provides clear, specific signals, essential for discerning NT5E expression levels across cell populations with high accuracy.
Q&As
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What are the primary research applications for Anti-Human NT5E Recombinant Antibody?
A: The antibody is mainly used in research focused on tumor microenvironment studies and immunotherapy strategies. It is suitable for applications like flow cytometry, immunohistochemistry, and ELISA, where it aids in understanding NT5E's role in cancer cell biology.
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Can this antibody help in studying diseases other than cancer?
A: Indeed, besides cancer, it's valuable in investigating autoimmune diseases and developmental biology, given NT5E's role in purine metabolism and cellular differentiation processes.
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What makes Oleclumab suitable for complex assays?
A: Oleclumab's Fab fragment format allows reduced steric hindrance, facilitating better antigen access in complex surface protein studies. This improves binding efficiency in assays like surface plasmon resonance.
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Does this antibody work with multiple species?
A: Although primarily designed for human NT5E, its cross-reactivity with similar isoforms in model organisms is currently under investigation, potentially extending its utility in comparative studies.
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What are the known applications in inflammation research?
A: In inflammation research, this antibody can be used to map NT5E expression in inflamed tissues, shedding light on how ecto-5'-nucleotidase modulates immune response and inflammation resolution.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-441CQ, RRID: AB_3111947)
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Biosimilar Overview
Please refer to Oleclumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Oleclumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1936z-F(E) | Recombinant Anti-Human NT5E Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| HPAB-N0374-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0374-YC-F(E)) | ELISA, FC, Inhib | Mouse Fab |
| HPAB-N0375-YC-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0375-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-M0172-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-M0172-YC-F(E)) | FC, Inhib | Mouse Fab |
| HPAB-M0173-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-M0173-YC-F(E)) | FC, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-593MZ | Human Anti-NT5E Recombinant Antibody (TAB-593MZ) | FC | Human IgG |
| TAB-594MZ | Human Anti-NT5E Recombinant Antibody (TAB-594MZ) | FC | Human IgG |
| TAB-595MZ | Human Anti-NT5E Recombinant Antibody (TAB-595MZ) | FC, FuncS | Human IgG |
| TAB-596MZ | Human Anti-NT5E Recombinant Antibody (TAB-596MZ) | FC | Human IgG |
| TAB-597MZ | Human Anti-NT5E Recombinant Antibody (TAB-597MZ) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1799CQ | Recombinant Mouse Anti-NT5E Antibody (NEUT-1799CQ) | FC, IHC-Fr, IF, IP, Inhib | IgG2a, κ |
| NEUT-1800CQ | Recombinant Rat Anti-Nt5e Antibody (TY/23) | BL | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2503 | Hi-Affi™ Recombinant Rabbit Anti-NT5E Monoclonal Antibody (DS2503AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0374-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0374-YC-S(P)) | ELISA, FC, Inhib | Mouse scFv |
| HPAB-N0375-YC-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0375-YC-S(P)) | ELISA, FC, Inhib | Human scFv |
| HPAB-M0172-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0172-YC-S(P)) | FC, Inhib | Mouse scFv |
| HPAB-M0173-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0173-YC-S(P)) | FC, FuncS | Mouse scFv |
| HPAB-N0196-YC-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0196-YC-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-441CQ | Afuco™ Anti-NT5E ADCC Recombinant Antibody, ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0500-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0500-FY) | Neut, FC, Block, FuncS, WB | Mouse IgG |
| HPAB-0501-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0501-FY) | Neut, FC, Block, FuncS, WB | Mouse IgG |
| HPAB-0502-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0502-FY) | Neut, FC, Block, FuncS, Inhib | Mouse IgG |
| HPAB-0503-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0503-FY) | Neut, FC, Block, FuncS | Mouse IgG |
| PABJ-0226 | Human Anti-NT5E Recombinant Antibody (PABJ-0226) | ELISA, Block | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1442 | AbPlus™ Anti-Nt5e Magnetic Beads (VS-0724-YC1442) | IP, Protein Purification | |
| VS-0724-YC1493 | AbPlus™ Anti-NT5E Magnetic Beads (VS-0724-YC1493) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC844 | CytoStream™ Mouse Anti-NT5E Recombinant Antibody (VS13-YC844) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY59 | Human Anti-NT5E (clone Uliledlimab) scFv-Fc Chimera | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC110 | Recombinant Anti-NT5E Vesicular Antibody, EV Displayed (VS-0425-YC110) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5002 | Anti-NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5003 | Anti-Mouse NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5004 | Anti-Rat NT5E Immunohistochemistry Kit | IHC |
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