Recombinant Mouse anti-Human RHOA Monoclonal antibody (1B3-4A10)
CAT#: MOB-0013CT
Recombinant Mouse monoclonal antibody to Human RHOA, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067560-RHOA
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Specifications
- Immunogen
- Recombinant full length protein, corresponding to amino acids 1-194 of Human RhoA
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 1B3-4A10
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.5 -1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- RHOA; ras homolog family member A; ARHA; ARH12; RHO12; RHOH12; transforming protein RhoA; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A;
- Gene ID
- 387
- UniProt ID
- P61586
- Protein Refseq
- NP_001655
- Function
- GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding;
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| FAMAB-0306WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0307WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone F7); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0308WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone D10); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0309WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone A5); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
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| FAMAB-0306WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0307WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone F7); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0308WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone D10); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0309WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone A5); Fab Fragment | ELISA, WB | Human Fab |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY6112 | Anti-Mouse RHOA Immunohistochemistry Kit | IHC | |
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