Recombinant Mouse anti-Human SMAD2 Monoclonal antibody (EML538) (CAT#: MOB-1885CT)
Recombinant Mouse monoclonal antibody to Human SMAD2, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
Specifications
- Immunogen
- Recombinant fragment: PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY , corresponding to amino acids 181-280 of Human Smad2
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML538
- Applications
- Used for immunoassay techniques such as: Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.5 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- SMAD2; SMAD family member 2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; mothers against decapentaplegic homolog 2; MAD homolog 2; mother against DPP homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2;
- Gene ID
- 4087
- UniProt ID
- Q15796
- Protein Refseq
- NP_001003652
- Function
- contributes_to DNA binding; I-SMAD binding; R-SMAD binding; SMAD binding; activating transcription factor binding; chromatin binding; co-SMAD binding; double-stranded DNA binding; phosphatase binding; protein binding; sequence-specific DNA binding transcr
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "SMAD2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4052z | Mouse Anti-SMAD2 Recombinant Antibody (clone 30G7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-2191-CN | Rabbit Anti-SMAD2 Polyclonal Antibody (MRO-2191-CN) | IF, IHC | Rabbit IgG |
| ZG-093R | Mouse Anti-Smad2 Recombinant Antibody (ZG-093R) | WB, ICC | Mouse IgG |
| VS3-QX1018 | Mouse Anti-SMAD2 Recombinant Antibody (clone 4D3) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-QX1019 | Mouse Anti-SMAD2 Recombinant Antibody (clone 6H5-E3-C11) | WB, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3321 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (DS3321AB) | FC, ICC, IHC-P, IP, WB | IgG |
| MOR-4512 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH20DS) | WB, IF, ICC | IgG |
| MOR-4709 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH223DS) | WB, IF, ICC, FC, ChIP | IgG |
| MOR-4735 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH249DS) | WB, IF, ICC, FC, ChIP, ELISA | IgG |
| MOR-0037-FY | Rabbit Anti-SMAD2 Recombinant Antibody (clone AFY0008) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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