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Recombinant Mouse anti-Human SMAD4 Monoclonal antibody (TNE57 (tbnf bt EDT-57)) (CAT#: MOB-0816CT)

Recombinant Mouse monoclonal antibody to Human SMAD4, expressed in Chinese Hamster Ovary cells(CHO).


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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_B_9_5.jpg
Figure 4 Colon
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_3.jpg
Figure 5 Liver
Figure 5 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_9_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_6_6.jpg
Figure 8 Placenta
Figure 8 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141646-SMAD4

Specifications

  • Immunogen
  • Purified recombinant DPC4 protein.
  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • TNE57 (tbnf bt EDT-57)
  • Applications
  • Used for immunoassay techniques such as: ChImmunoprecipitation; Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • Protein G purified
  • Format
  • Liquid
  • Concentration
  • 0.2 mg/ml
  • Buffer
  • Preservative: 0.09% Sodium Azide Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Storage
  • Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Target

  • Alternative Names
  • SMAD4; SMAD family member 4; JIP; DPC4; MADH4; MYHRS; mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4;
  • Function
  • contributes_to DNA binding; DNA binding; I-SMAD binding; R-SMAD binding; SMAD binding; chromatin binding; collagen binding; core promoter proximal region sequence-specific DNA binding; identical protein binding; protein binding; contributes_to protein bin

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "SMAD4"

Recombinant Antibody

CAT Product Name Application Type
MOB-2804z Mouse Anti-SMAD4 Recombinant Antibody (clone 22G1) WB, ELISA, FC, IF, IHC Mouse IgG
MRO-1405-CN Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (CBACN-513) WB, IF, IHC, FC Rabbit IgG
VS3-QX1028 Mouse Anti-SMAD4 Recombinant Antibody (clone 5B8-F7-F3) WB, IHC, ICC, FC, ELISA Mouse IgG1
VS3-WK935 Rabbit Anti-SMAD4 Recombinant Antibody (VS3-WK935) WB, ICC, IF, IHC, FC Rabbit IgG
VS4-CJ119 Recombinant Rabbit Anti-SMAD4 Antibody (clone 22H12) ELISA, WB Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3323 Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (DS3323AB) WB, IHC-P, ICC, FC, IP IgG
MOR-4736 Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (TH250DS) WB, IF, ICC, FC IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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