Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2)
CAT#: MOB-2017CT
Recombinant Mouse monoclonal antibody to Human TGFA, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/476_C8_2_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HBEC3-KT, OE19, RPTEC TERT1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163235-TGFA
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Specifications
- Immunogen
- Synthetic peptide fragment (32-40 amino acids sequence of 50 amino acids) of transforming growth factor alpha (TGF-alpha).
- Host Species
- Mouse
- Specificity
- This antibody was selected and evaluated immunoserologically for recombinant human TGF-alpha and synthetic (uncoupled) peptides using simple binding and competitive ELISA.Immunohistochemical characterization of the anti-TGF-alpha monoclonal antibody was m
- Species Reactivity
- Human
- Clone
- Q/U2
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Immunocytochemistry; Immunohistochemistry (Frozen sections); Immunohistochemistry paraffin embedded sections
- Conjugate
- Unconjugated
Product Property
- Purity
- IgG fraction
- Format
- Liquid
- Concentration
- 0.2 mg/ml
- Buffer
- Preservative: 15mM Sodium Azide Constituents: Tris HCl, 2mg/ml BSA
- Storage
- Store at +4°C.
Target
- Alternative Names
- TGFA; transforming growth factor, alpha; TFGA; protransforming growth factor alpha; TGF-alpha;
- Gene ID
- 7039
- UniProt ID
- P01135
- Protein Refseq
- NP_001093161
- Function
- MAP kinase kinase activity; epidermal growth factor receptor binding; glycoprotein binding; growth factor activity; protein binding; signal transducer activity;
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Related Diseases
Non-small Cell Lung Cancer
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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