Recombinant Anti-Human chorionic gonadotropin hormone (hCG) alpha subunit VHH Single Domain Antibody (H14)

Figure 1 Antigen binding after temperature treatment of hCG specific llama VHHs.

Antibodies were incubated for 2 h at di¡erent temperatures, cooled down to room temperature and subsequently ELISA was performed.

Van der Linden, R. H. J., Frenken, L. G. J., De Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., ... & Verrips, C. T. (1999). Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1431(1), 37-46.

Figure 2 Antigen binding at 90℃ of llama VHHs and mouse mAbs specific for RR-6 as measured by ELISA.

The antigen binding at 90°C was performed in the absence (E) or presence of 1 WM RR-6 (F) to discriminate speci¢c from aspecific binding.

Van der Linden, R. H. J., Frenken, L. G. J., De Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., ... & Verrips, C. T. (1999). Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1431(1), 37-46.

Figure 3 Antigen binding in the presence of ammonium thiocyanate at subsaturation point of hCG specific mouse llama VHHs.

Van der Linden, R. H. J., Frenken, L. G. J., De Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., ... & Verrips, C. T. (1999). Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1431(1), 37-46.

Figure 4 Antigen binding in the presence of ethanol at subsaturation point of hCG specific mouse llama VHHs.

Van der Linden, R. H. J., Frenken, L. G. J., De Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., ... & Verrips, C. T. (1999). Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1431(1), 37-46.