Recombinant Human Anti-IAV HA Antibody (65C6) (CAT#: PABL-208)

Recombinant Human Antibody (65C6) is capable of binding to IAV HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

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Figure 1 Bound antibodies 65C6 and 100F4 are internalized and block low-pH-induced fusion.

Figure 1 Bound antibodies 65C6 and 100F4 are internalized and block low-pH-induced fusion.

(C) Blockage of low-pH-triggered, wild-type H5 HA-mediated fusion by antibodies 65C6 and 100F4 but not PG16 control. Mean and standard deviation of fusion percentage in each image is shown below. (D) Blockage of low-pH-triggered, escape A of H5 HA-mediated fusion by antibody 65C6 but not by antibody 100F4 and PG16 control.

Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.

Figure 2 Neutralization activity against H5N1 pseudotype A/Shenzhen/406H/06 by indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16. The neutralization activity was calculated as follows: relative luciferase activity (RLA) of pseudotype with a given concentration of an antibody/RLA of pseudotype alone without antibodies.

Figure 2 Neutralization activity against H5N1 pseudotype A/Shenzhen/406H/06 by indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16. The neutralization activity was calculated as follows: relative luciferase activity (RLA) of pseudotype with a given concentration of an antibody/RLA of pseudotype alone without antibodies.

Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.

Figure 3 (B) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on binding of H5N1 pseudotype. A/Shenzhen/406H/06 to MDCK cells compared to binding of pseudotype alone (without antibodies). (C) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on postattachment entry of H5N1 pseudotype A/Shenzhen/406H/06 into MDCK cells compared to entry of pseudotype alone (without antibodies).

Figure 3 (B) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on binding of H5N1 pseudotype. A/Shenzhen/406H/06 to MDCK cells compared to binding of pseudotype alone (without antibodies). (C) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on postattachment entry of H5N1 pseudotype A/Shenzhen/406H/06 into MDCK cells compared to entry of pseudotype alone (without antibodies).

Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.

Figure 4 (D) Effects of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on pH 5.0-triggered, HA-mediated cell-cell fusion among influenza H5 HA-transduced HeLa cells. (E) Mean and standard deviation of fusion percentages in each image in panel D.

Figure 4 (D) Effects of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on pH 5.0-triggered, HA-mediated cell-cell fusion among influenza H5 HA-transduced HeLa cells. (E) Mean and standard deviation of fusion percentages in each image in panel D.

Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.


Specifications

  • Immunogen
  • Influenza a virus Hemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native IAV HA
  • Species Reactivity
  • IAV
  • Clone
  • 65C6
  • Applications
  • WB, ELISA, Neut, Block, FuncS

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody was validated for Block, Neutralization and Function Assay. For details, refer to Published Data.

Target

  • Alternative Names
  • HA; hemagglutinin; haemagglutinin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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