Recombinant Human Anti-IAV HA Antibody (65C6) (CAT#: PABL-208)
Recombinant Human Antibody (65C6) is capable of binding to IAV HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Bound antibodies 65C6 and 100F4 are internalized and block low-pH-induced fusion.
(C) Blockage of low-pH-triggered, wild-type H5 HA-mediated fusion by antibodies 65C6 and 100F4 but not PG16 control. Mean and standard deviation of fusion percentage in each image is shown below. (D) Blockage of low-pH-triggered, escape A of H5 HA-mediated fusion by antibody 65C6 but not by antibody 100F4 and PG16 control.
Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.
Figure 2 Neutralization activity against H5N1 pseudotype A/Shenzhen/406H/06 by indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16. The neutralization activity was calculated as follows: relative luciferase activity (RLA) of pseudotype with a given concentration of an antibody/RLA of pseudotype alone without antibodies.
Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.
Figure 3 (B) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on binding of H5N1 pseudotype. A/Shenzhen/406H/06 to MDCK cells compared to binding of pseudotype alone (without antibodies). (C) Effects of indicated concentrations of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on postattachment entry of H5N1 pseudotype A/Shenzhen/406H/06 into MDCK cells compared to entry of pseudotype alone (without antibodies).
Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.
Figure 4 (D) Effects of antibodies 65C6, 100F4, AVIg03, F10, and PG16 on pH 5.0-triggered, HA-mediated cell-cell fusion among influenza H5 HA-transduced HeLa cells. (E) Mean and standard deviation of fusion percentages in each image in panel D.
Qian, M., Hu, H., Zuo, T., Wang, G., Zhang, L., & Zhou, P. (2013). Unraveling of a neutralization mechanism by two human antibodies against conserved epitopes in the globular head of H5 hemagglutinin. Journal of virology, 87(6), 3571-3577.
Specifications
- Immunogen
- Influenza a virus Hemagglutinin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native IAV HA
- Species Reactivity
- IAV
- Clone
- 65C6
- Applications
- WB, ELISA, Neut, Block, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for Block, Neutralization and Function Assay. For details, refer to Published Data.
Target
- Alternative Names
- HA; hemagglutinin; haemagglutinin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
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