Recombinant Human Antibody (C05) is capable of binding to IAV HA1 chain, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA1 chain mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA1 chain mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 EC50 measurements with ELISA for the IgGs reformatted from the selected anti-HA trimer scFvs.
Chen, C., Chiu, Y. K., Yu, C. M., Lee, C. C., Tung, C. P., Tsou, Y. L.,... & Yang, A. S. (2017). High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries. Scientific reports, 7(1), 14455.
Figure 2 IC50 measurements with pseudo virus-based microneutralization assay for the IgGs reformatted from the selected anti-HA trimer scFvs.
Chen, C., Chiu, Y. K., Yu, C. M., Lee, C. C., Tung, C. P., Tsou, Y. L.,... & Yang, A. S. (2017). High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries. Scientific reports, 7(1), 14455.
Figure 3 C05 protects mice from lethal virus challenge.
Survival and weight loss were monitored in response to varying amounts of C05 IgG administered prophylactically to mice 24 hours before challenge with (a) 25x the 50% mouse lethal dose (MLD50) of A/Memphis/3/2008 (H1N1) or (b) 33 MLD50 of A/Aichi/2/X-31/1968 (H3N2) viruses. A single therapeutic dose of 15 mg/kg C05 IgG was delivered 1, 2, 3, 4, or 5 days post-challenge with (c) 25 MLD50 of A/Memphis/3/2008 (H1N1) or (d) 33 MLD50 of A/Aichi/2/X-31/1968 (H3N2) viruses.
Ekiert, D.C., Kashyap, A.K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R.,...& Wilson, A.I. (2012). Neutralization of influenza A viruses by insertion of a single antibody loop into the receptor binding site. Nature, 489(7417), 526–532.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-203 | Recombinant Human Anti-IAV HA Antibody (3.1) | WB, ELISA, FuncS | IgG |
PABL-204 | Recombinant Human Anti-IAV HA Antibody (39.29) | Neut, FuncS | IgG |
PABL-205 | Recombinant Human Anti-IAV HA Antibody (1957) | WB, Neut, FuncS | IgG |
PABL-206 | Recombinant Human Anti-IAV HA Antibody (1F1) | WB, ELISA, FuncS | IgG |
PABL-207 | Recombinant Human Anti-IAV HA Antibody (5J8) | FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBC-160 | Human Anti-HA Recombinant Antibody; Fab Fragment (PFBC-160) | Neut, ELISA, FC | Human Fab |
HPAB-M0099-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (Ab 044) | ELISA, FuncS | Human scFv |
HPAB-M0100-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (FI6) | ELISA, FuncS | Human scFv |
HPAB-M0101-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (Ab 032) | ELISA, FuncS | Human scFv |
HPAB-0928WJ-S(P) | Recombinant Mouse Anti-HA Antibody scFv Fragment (3C8) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-268CT | Anti-IAV HA Recombinant Antibody (D6-1) | WB, FC, IP | Human antibody |
MRO-269CT | Anti-IAV HA Recombinant Antibody (IgA 59) | WB, Neut | Human antibody |
MRO-270CT | Anti-IAV HA Recombinant Antibody (IgA 119) | WB, Neut | Human antibody |
MRO-271CT | Anti-IAV HA Recombinant Antibody (IgA 340) | WB, Neut | Human antibody |
MRO-272CT | Anti-IAV HA Recombinant Antibody (IgG 134) | WB, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-307CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (8982) | WB, ELISA | |
MRO-308CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (8153) | WB, ELISA | |
MRO-309CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-7) | WB, ELISA | |
MRO-310CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-5) | WB, ELISA | |
MRO-311CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-17) | WB, ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-070-S (P) | Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4209 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI287DS) | ELISA, ICC/IF, IF, FC | IgG |
MOR-4210 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI288DS) | ELISA, ICC/IF, IF, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1169-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1169-FY) | Neut | Llama VHH |
HPAB-1170-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1170-FY) | Neut | Llama VHH |
HPAB-1171-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1171-FY) | Neut | Llama VHH |
HPAB-1172-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1172-FY) | Neut | Llama VHH |
HPAB-1173-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1173-FY) | Neut | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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