Recombinant Mouse Anti-IAV M2 protein Antibody (mAb 65)
CAT#: PABL-226
Recombinant Mouse Antibody (mAb 65) is capable of binding to IAV M2 protein, expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Tested Data
Figure 1 Reactivity of MAb 65 and 14C2 with M2 was analyzed by Western blotting of lysates of HEK-293T cells transfected with M2-Flag expressionconstructs carrying M2e-Ala mutants. Anti-Flag detection was used to normalize the amount of M2 in the cell lysates that were loaded. NC, negative control.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 2 Confocal images of immunofluorescence staining of MDCK cells infected with in-fluenza virus.
M2 staining is shown in red fluorescence and was revealed usingMAb 65 or MAb 148 (a TCN-031-like antibody that binds to the N terminus ofM2). Goat polyclonal antibody against influenza virus ribonucleoprotein (BEIResources, no. NR-3133) was used to stain viral RNPs (green). Cell nuclei were identified by DAPI staining (blue).
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 3 MAb 65 does not affect influenza A virus plaque size.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 4 Properties of binding of MAb 65 to M2e peptide and M2-expressing cells.
Binding of MAb 65 and MAb 148 to different M2e peptides. ELISA plateswere coated with wild-type (WT), M2eW15G, or M2eP10L M2e peptides at 20 ng/ml or 200 ng/ml.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 5 Cellular ELISA using HEK-293T cells that had been transfected with wild-type, M2Trp15Ala, or M2Pro10Ala M2-Flag expression vectors.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 6 Western blot analysis of cell lysates prepared 24 h after transfection to evaluateexpression of NP, M2 (revealed by staining with MAb 148 and MAb 65) and M1, as indicated. WT, wild type; NC, negative control (i.e., transfected with only pCAXL-PA, pCAXL-PB1, pCAXL-PB2, and pCAXL-NP); ST, mutant with stop codons introduced after M2-Asp24; NT, nontransfected.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 7 Plaque assay toquantify rescued PR/8 viruses with introduced M1/M2Trp15 mutations.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
Figure 8 Hemagglutinating activity in the supernatants of transfected HEK293T-MDCKcocultures. The supernatant and serial 3-fold dilutions were assayed for hemagglutination of chicken red blood cells on day 2 or 5 after transfection with theeight-plasmid system used for generating PR/8 virus.
Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H.,... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.
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WB
Figure 1 Anti-IAV M2 Antibody (mAb 65) (PABL-226) in WB
Western blot analysis of PABL-226 was performed with IAV M2 Protein (His Tag). PABL-226 incubation concentration: 2 ng/μL. The secondary antibody: HRP-Anti-mouse IgG (H+L) Lane 1: Reducing Antigen (0.1μg) Lane 2: Reducing Antigen (0.3μg) Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Anti-IAV M2 Antibody (mAb 65) (PABL-226) in ELISA
ELISA analysis of PABL-226 was performed with IAV M2 Protein (His Tag). The secondary antibody: HRP-goat-Anti-mouse IgG (H+L)
DB
Figure 3 Anti-IAV M2 Antibody (mAb 65) (PABL-226) in Dot Blot
Dot Blot analysis of PABL-226 was performed with IAV M2 Protein (His Tag). PABL-226 incubation concentration: 2 ng/μL. The secondary antibody: HRP-Anti-mouse IgG (H+L)
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Specifications
- Immunogen
- Influenza a virus matrix protein 2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native IAV M2 protein
- Species Reactivity
- IAV
- Clone
- mAb 65
- Applications
- IF, FuncS, ELISA, WB, Plaque assay, Hemagglutinating activity
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA, Immunofluorescence, Western Blot, Plaque assay, Function Assay and Hemagglutinating activity. For details, refer to Published Data.
Target
- Alternative Names
- M2 protein; matrix protein 2; M2e
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-226 | Recombinant Mouse Anti-IAV M2 protein Antibody scFv Fragment (mAb 65) | WB, ELISA, FuncS | scFv |
| HPAB-AP732-YC-S(P) | Recombinant Humanized Anti-M2 protein Antibody scFv Fragment (h14C2) | ELISA, FC, Inhib, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-226 | Recombinant Mouse Anti-IAV M2 protein Antibody Fab Fragment (mAb 65) | WB, ELISA, FuncS | Fab |
| HPAB-AP732-YC--F(E) | Recombinant Humanized Anti-M2 protein Antibody Fab Fragment (h14C2) | ELISA, FC, Inhib, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-240CT | Anti-IAV M2 Recombinant Antibody (14C2) | WB | |
| MRO-240CT-S(P) | Anti-IAV M2 Recombinant Antibody scFv Fragment (14C2) | IF, ELISA | |
| MRO-240CT-F(E) | Anti-IAV M2 Recombinant Antibody Fab Fragment (14C2) | IF, ELISA |
| CAT | Product Name | Application | Type |
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| MOB-1777CT | Recombinant Mouse anti-Respiratory Syncytial Virus M2 Monoclonal antibody (STW6I6) | ELISA, ICC, IF, IP, WB | |
| HPAB-AP732-YC | Recombinant Humanized Anti-M2 protein Antibody (h14C2) | FC, WB, ELISA, FC, Inhib | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0173 | APC-A*02:01/M2 (LMLGEFLKL) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
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