Mouse Anti-ICAM1 Recombinant Antibody (clone CA7) (CAT#: PABZ-079)

Recombinant Mouse Antibody (CA7) is capable of binding to ICAM-1, expressed in Chinese Hamster Ovary cells (CHO). CA7 antibody binds to monomeric but not dimeric ICAM-1.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 ELISA

Figure 1 ELISA

Purified ICAM-1 prepa- ons (500 ng/ml) were tested for binding to immobilized R6.5, CA7, or 2H 11 b. Bound ICAM-1 was detected with biotin-conjugated-CBRIC1/11 mAb owed by streptavidin-conjugated horseradish peroxidase

Jun, C. D., Shimaoka, M., Carman, C. V., Takagi, J., & Springer, T. A. (2001). Dimerization and the effectiveness of ICAM-1 in mediating LFA-1-dependent adhesion. Proceedings of the National Academy of Sciences, 98(12), 6830-6835.

Figure 2 Differential ICAM-1 epitope expression on normal cells and ICAM-1 transfected cells.

Figure 2 Differential ICAM-1 epitope expression on normal cells and ICAM-1 transfected cells.

Binding of mouse mAbs TS2/18 (negative control, solid) RR1/1 (dotted), CA7 (stippled), and M261PP5C7 (dashed) was determined by subsequent binding of FITC goat anti-mouse IgG and flow microfluorimetry on a FACSan® (Becton Dickinson and Co.). Cells tested were (A) JY B lymphoblasts; (B) BHK-VP16 cells transfected with transmembrane ICAM-1; and (C) BHK-VP16 cells transfected with GPI-ICAM-1.

Miller, J., Knorr, R., Ferrone, M., Houdei, R., Carron, C. P., & Dustin, M. L. (1995). Intercellular adhesion molecule-1 dimerization and its consequences for adhesion mediated by lymphocyte function associated-1. Journal of Experimental Medicine, 182(5), 1231-1241.


Specifications

  • Immunogen
  • Human intercellular adhesion molecule 1
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human ICAM1
  • Species Reactivity
  • Human
  • Clone
  • CA7
  • Applications
  • FC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody CA7 has been reported in applications of ELISA, FC. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • ICAM1; intercellular adhesion molecule 1; BB2; CD54; P3.58; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Immunotoxin

CAT Product Name Application Type
AGTO-L023R anti-ICAM1 immunotoxin UV3 (IgG)-RTA Cytotoxicity assay, Functional assay

Humanized Antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-104LC Recombinant Anti-Human ICAM1 Antibody (Fc glycosylation) ELISA Mouse antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-923CQ Mouse Anti-ICAM1 Recombinant Antibody (clone CBL236) Neut, IF, FC, WB, IHC Mouse IgG1
NEUT-925CQ Mouse Anti-ICAM1 Recombinant Antibody (clone HA58) FC, FuncS, IHC, Neut, WB Mouse IgG1, κ
NEUT-926CQ Mouse Anti-ICAM1 Recombinant Antibody (VMC182) FC, IF, IHC, ICC, Neut Mouse IgG2b
NEUT-927CQ Mouse Anti-ICAM1 Recombinant Antibody (clone RR1/1) FC, IF, IHC, Neut, WB, ELISA Mouse IgG1
NEUT-930CQ Rat Anti-Icam1 Recombinant Antibody (clone KAT1) FC, FuncS, IHC-Fr, Neut, IP Rat IgG2a, κ

Blocking Antibody

CAT Product Name Application Type
NEUT-924CQ Mouse Anti-ICAM1 Recombinant Antibody (clone HCD54) FC, IF, Block Mouse IgG1, κ
NEUT-928CQ Mouse Anti-ICAM1 Recombinant Antibody (VMC183) Block, FC, IHC, IP Mouse IgG1
NEUT-929CQ Mouse Anti-ICAM1 Recombinant Antibody (clone BBIG-I1(11C81)) Block, WB, IP, ICC Mouse IgG1

Rabbit Monoclonal Antibody

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-1635LC Mouse Anti-ICAM1 Recombinant Antibody (clone c78.4A) FC Mouse IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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