Mouse Anti-IFNA2 Recombinant Antibody (clone 3B7) (CAT#: HPAB-0116-LSX)
Recombinant monoclonal antibody 3B7 is a IgG2a that against the INFAR2 binding of various type I interferons.
Specific Inquiry
Figure 1 IFN-e has growth inhibitory effect on Daudi cells.
Daudi cells (4 x lO Vml) were treated with IFN-e of various concentrations in the presence or absence of 0.5 μg/ml of monoclonal antibodies anti-IFN-αRl (2E1), anti-IFN- αR2 (3B7) or control antibody anti-HER2 for 72 hours. Cell proliferation was examined by AlamarBlue assay. At least three experiments were performed and a representative result is shown.
WB
Figure 1 Immunoprecipitation of the 130-kDa complexes in affinity crosslinked material by mAbs directed against the IFNa receptor.
Nonimmune mouse IgG1 (Coulter) was used as a control.
Colamonici, O. R., D'Alessandro, F., Diaz, M. O., Gregory, S. A., Neckers, L. M., & Nordan, R. (1990). Characterization of three monoclonal antibodies that recognize the interferon alpha 2 receptor. Proceedings of the National Academy of Sciences, 87(18), 7230-7234.
FC
Figure 2 Flow cytometric analysis of U-266 cells with the anti-IFNa receptor mAb.
The data was analyzed in an Epics cell sorter (Coulter).
Colamonici, O. R., D'Alessandro, F., Diaz, M. O., Gregory, S. A., Neckers, L. M., & Nordan, R. (1990). Characterization of three monoclonal antibodies that recognize the interferon alpha 2 receptor. Proceedings of the National Academy of Sciences, 87(18), 7230-7234.
Specifications
- Immunogen
- hIFNAR2-IgG fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human IFNA2
- Species Reactivity
- Human
- Clone
- 3B7
- Applications
- WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The IFNA2 antibody has been reported in applications of Western Blot, Flow Cytometry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1175z | Mouse Anti-IFNA2 Recombinant Antibody (clone 33D4) | ELISA, FC, ICC, IF | Mouse IgG1, κ |
| MOB-2029MZ | Recombinant Mouse Anti-Human IFNA2 Antibody (clone O38) | ELISA, ELISA, WB | Mouse antibody |
| HPAB-0117-LSX | Mouse Anti-IFNA2 Recombinant Antibody (clone 1F3) | WB | Mouse IgG2a |
| HPAB-0118-LSX | Mouse Anti-IFNA2 Recombinant Antibody (clone 1D3) | WB | Mouse IgG2a |
| HPAB-1731-FY | Mouse Anti-IFNA2 Recombinant Antibody (clone ScFv17) | ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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